Human PMEL Antibody

Catalog #: MAB11439 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB11439-SP	Ships in 5-7 Days	25 ug / $159
MAB11439-100	In Stock	100 ug / $449

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Human PMEL Antibody Summary

Species Reactivity

Human

Specificity

Detects human PMEL in direct ELISA.

Source

Monoclonal Mouse IgG_2B Clone # 1067144

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese Hamster Ovary cell line, CHO-derived human PMEL
Lys25-Gln595
Accession # P40967

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

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Applications

Recommended Concentration

Sample

Immunohistochemistry

5-15 µg/mL

Immersion fixed paraffin-embedded sections of Human Melanoma

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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Detection of PMEL in Human Melanoma. PMEL was detected in immersion fixed paraffin-embedded sections of Human Melanoma using Mouse Anti-Human PMEL Monoclonal Antibody (Catalog # MAB11439) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PMEL

Premelanasome protein (PMEL) is a nonmutated "self" antigen expressed by melanocytes, most melanoma cells, substantia nigra, and retinal pigmented epithelium. This protein plays an essential role in the structural organization of premelanosomes. PMEL is over-expressed in all stages of melanoma progression and is negative prognostic marker for the overall survival rate of melanoma patients. PMEL is also associated with melanin-based pigmentation, neurodegenerative disease (functional amyloid) and glaucoma (missense mutations). How PMEL amyloid is tolerated within cells is of major therapeutic interest as it is related to Alzheimer's and Parkinson's disease.

References

Zhang, S. Chen, K. Liu, H. Jing, C. Zhang, X. Qu, C. Yu, S. PMEL as a Prognostic Biomarker and Negatively Associated With Immune Infiltration in Skin Cutaneous Melanoma (SKCM). J. Immunother. 2021 Jul-Aug 01;44(6):214-223.
doi: 10.1097/CJI.0000000000000374. PMID: 34028390; PMCID: PMC8225232.
Fan P.-D. Goff S.P. Mol. Cell. Biol. 20:7591-7601(2000). Chrystal, P.W. Footz, T. Hodges, E.D. Jensen, J.A. Walter, M.A. Allison, W.T. Functional Domains and Evolutionary History of the PMEL and GPNMB Family Proteins. Molecules. 2021 Jun 9;26(12):3529.
doi: 10.3390/molecules26123529. PMID: 34207849; PMCID: PMC8273697.

Long Name

Premelanosome Protein

Entrez Gene IDs

6490 (Human); 20431 (Mouse)

Alternate Names

D12S53EP1; Gp100; ME20; ME20M; ME20-M; melanocyte protein mel 17; Melanocyte protein Pmel 17; Melanocytes lineage-specific antigen GP100; Melanoma-associated ME20 antigen; melanosomal matrix protein17; PMEL; PMEL17; PMEL17P100; premelanosome proteinME20M; SI; SIL; SILV; silver (mouse homolog) like; silver homolog (mouse); Silver locus protein homolog; silver, mouse, homolog of; SILVPmel17