Key Product Details

Species Reactivity

Human

Applications

Western Blot, Simple Western

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all PON1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human PON1
Ala30-Leu355
Accession # P27169

Specificity

Detects human PON1 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human PON1 Antibody

Detection of Human PON1 antibody by Western Blot.

Detection of Human PON1 by Western Blot.

Western blot shows lysates of human liver tissue. PVDF membrane was probed with 0.5 µg/mL Goat Anti-Human PON1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5816) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). For additional reference, an enriched HDL/LDL fraction obtained from ultracentrifugation of human plasma was included. A specific band for PON1 was detected at approximately 43 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Detection of Human PON1 antibody by Simple WesternTM.

Detection of Human PON1 by Simple WesternTM.

Simple Western lane view shows lysates of human liver tissue, loaded at 0.2 mg/mL. A specific band was detected for PON1 at approximately 52 kDa (as indicated) using 5 µg/mL of Goat Anti-Human PON1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5816) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human PON1 Antibody

Application
Recommended Usage

Simple Western

5 µg/mL
Sample: Human liver tissue

Western Blot

0.5 µg/mL
Sample: Human liver tissue

Reviewed Applications

Read 1 review rated 5 using AF5816 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PON1

The paraoxonase (PON) gene family of antioxidant enzymes includes three known members located adjacent to each other on chromosome 7. Paraoxonase/arylesterase 1 (PON1, also known as serum paraoxonase) is a 355 amino acid, 43 kDa glycoprotein that is expressed in liver and is secreted into the bloodstream where it associates with high-density lipoproteins (HDL). Serum PON1 concentrations vary widely among normal individuals, in part due to differential expression of some polymorphisms. Sequence polymorphisms in this gene may be associated with coronary heart disease and a number of phenotypes related to diabetes. PON1 is primarily a lactonase (EC 3.1.8.1) that is thought to attenuate the oxidation of low-density lipoproteins (LDL). This may slow the initiation and progression of atherosclerosis. Human PON1 shares 83% and 81% aa identity with mouse and rat PON1, respectively.

Long Name

Paraoxonase 1

Alternate Names

ESA, K-45

Entrez Gene IDs

5444 (Human); 18979 (Mouse); 84024 (Rat)

Gene Symbol

PON1

UniProt

P27169 (Human)

Additional PON1 Products

Product Documents for Human PON1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human PON1 Antibody

For research use only

Related Research Areas

Cancer Stem Cell Markers

Customer Reviews for Human PON1 Antibody (1)

5 out of 5
1 Customer Rating
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  • Human PON1 Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Purified Standard
    Species: Human
    Verified Customer | Posted 12/08/2017

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