Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Intracellular Staining by Flow Cytometry, CyTOF-ready

Cited:

Luminex Development

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 393005
View all RBP4/Retinol-Binding Protein 4 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human RBP4/Retinol‑Binding Protein 4
Glu19-Leu201
Accession # P02753

Specificity

Detects human RBP4/Retinol‑Binding Protein 4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, 25% cross-reactivity with recombinant mouse RBP4 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human RBP4/Retinol‑Binding Protein 4 Antibody

Detection of RBP4/Retinol-Binding Protein 4 antibody in HepG2 Human Cell Line antibody by Flow Cytometry.

Detection of RBP4/Retinol-Binding Protein 4 in HepG2 Human Cell Line by Flow Cytometry.

HepG2 human hepato-cellular carcinoma cell line was stained with Mouse Anti-Human RBP4/Retinol-Binding Protein 4 Monoclonal Antibody (Catalog # MAB3378, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with methanol.

Applications for Human RBP4/Retinol‑Binding Protein 4 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

2.5 µg/106 cells
Sample: HepG2 human hepatocellular carcinoma cell line fixed with paraformaldehyde and permeabilized with methanol

Western Blot

1 µg/mL
Sample: Recombinant Human RBP4/Retinol-Binding Protein 4 (Catalog # 3378-LC)

Flow Cytometry Panel Builder

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Advanced Features

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  • Spillover Popups - Visualize the spectra of individual fluorochromes
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: RBP4/Retinol-Binding Protein 4

Retinol (also known as vitamin A) is unstable and insoluble in the aqueous solution (1, 2). However, retinol becomes quite stable and soluble in plasma due to its tight interaction with retinol-binding protein 4 (RBP4), also known as plasma retinol-binding protein. A prototypic member of the lipocalin superfamily, RBP4 has a beta -barrel structure with a well-defined cavity. It is secreted from the liver, a process requiring the availability of retinol. RBP4 delivers retinol from the liver to the peripheral tissues. In plasma, the RBP4-retinol complex interacts with transthyretin (TTR), also known as thyroxine-binding protein and prealbumin. The retinol-RBP4-TTR complex prevents the loss of RBP4 by filtration through the kidney and increases the stability of the retinol-RBP4 complex. Defects in RBP4 cause retinol-binding protein deficiency, which affects night vision.

References

  1. Zanotti, G. and R. Berni. (2004) Vitamins and Hormones 69:271.
  2. Newcomer, M.E. and D.E. Ong. (2000) Biochim. Biophys. Acta 1482:57.

Alternate Names

RetinolBinding Protein 4

Entrez Gene IDs

5950 (Human); 19662 (Mouse); 25703 (Rat)

Gene Symbol

RBP4

UniProt

P02753

Additional RBP4/Retinol-Binding Protein 4 Products

Product Documents for Human RBP4/Retinol‑Binding Protein 4 Antibody

Certificate of Analysis

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Product Specific Notices for Human RBP4/Retinol‑Binding Protein 4 Antibody

For research use only

Citations for Human RBP4/Retinol‑Binding Protein 4 Antibody

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FAQs

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Associated Pathways

Adipokines & Insulin Signaling Pathways Adipokines & Insulin Signaling Pathway Thumbnail