Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Rat

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Western Blot, Flow Cytometry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all SMAC/Diablo Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human SMAC/Diablo
Ala56-Asp239
Accession # Q9NR28

Specificity

Detects human, mouse, and rat SMAC/Diablo in direct ELISAs and Western blots. No cross-reactivity with recombinant human NME3 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human SMAC/Diablo Antibody

SMAC/Diablo antibody in Human Prostate by Immunohistochemistry (IHC-P).

SMAC/Diablo in Human Prostate.

SMAC/Diablo was detected in immersion fixed paraffin-embedded sections of human prostate using Goat Anti-Human SMAC/Diablo Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7891) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of glandular epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Human SMAC/Diablo antibody by Western Blot.

Detection of Human SMAC/Diablo by Western Blot.

Western blot shows lysates of SH-SY5Y human neuroblastoma cell line, mouse NB41A3 mouse neuroblastoma cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human SMAC/Diablo Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7891) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for SMAC/Diablo at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Applications for Human SMAC/Diablo Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human kidney and prostate

Western Blot

0.5 µg/mL
Sample: SH-SY5Y human neuroblastoma cell line, mouse NB41A3 mouse neuroblastoma cell line, and PC-12 rat adrenal pheochromocytoma cell line,

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SMAC/Diablo

SMAC (second mitochondria derived activator of caspase)/Diablo promotes caspase activation by interacting with the inhibitor of apoptosis (IAP) proteins in the cytochrome c/Apaf-1/caspase-9 pathway.

References

  1. Du, C. et al. (2000) Cell 102:33.
  2. Chai, J. et al. (2000) Nature 406:855.
  3. Srinivasula, S. et al. (2000) J. Biol. Chem. 275:36152.
  4. Ekert, P. et al. (2001) J. Cell Biol. 152:483.
  5. Verhagen, A. (2000) Cell 102:43.

Long Name

Second Mitochondria-derived Activator of Caspase

Alternate Names

Diablo

Entrez Gene IDs

56616 (Human)

Gene Symbol

DIABLO

UniProt

Q9NR28

Additional SMAC/Diablo Products

Product Documents for Human SMAC/Diablo Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human SMAC/Diablo Antibody

For research use only

Related Research Areas

Bcl-2 Proteins and Regulators

Citations for Human SMAC/Diablo Antibody

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Protocols

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