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Human SMPD1 Antibody

Catalog #: MAB5348
2 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB5348
MAB5348-SP
Product Details
Citations (2)
FAQs
Supplemental Products
Reviews (1)
Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human SMPD1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human SMPD1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human SMPD3 and recombinant mouse SMPD1 is observed.
Source
Monoclonal Mouse IgG2A Clone # 563418
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human SMPD1 isoform 1
His62-Pro628
Accession # NP_000534
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human SMPD1
(Catalog # 5348-PD), see our available Western blot detection antibodies.
Neutralization
Measured by its ability to neutralize Recombinant Human SMPD1 (0.5 µg/mL, Catalog # 5348-PD) cleavage of the substrate 2-N-Hexadecanoylamino- 4-nitrophenylphosphorylcholine (HNPPC, 250 µM). The Neutralization Dose (ND50) is typically 3.0 µg/mL

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SMPD1

Sphingomyelin phosphodiesterase, also known as acid sphingomyelinase and encoded by the SMPD1 gene, is a lysosomal phosphodiesterase which belongs to the acid sphingomyelinase family (1). SMPD1 catalyzes the hydrolysis of sphingomyelin to ceramide and phosphorylcholine. Ceramide, a bioactive lipid, has emerged as an important signaling molecule involved in a variety of cellular processes such as cell differentiation, apoptosis, and proliferation (2). Activation of SMPD1 occurs by the removal, chemical modification or dimerization of its C-terminal cysteine residue (3). Deficiencies of SMPD1 result in a lysosomal storage disorder referred to as Niemann-Pick disease (4). rhSMPD1 was expressed without the last three C-terminal residues, and is therefore constitutively active.

References
  1. Schuchman, E.H. et al. (1991) J. Biol. Chem. 266:8531.
  2. Melendez, A.J. et al. (2008) Biochim. Biophys. Acta 1784:66.
  3. Qiu, H. et al. (2003) J. Biol. Chem. 278:32744.
  4. Smith, E.L. and Schuchman, E.H. (2008) FASEB J. 22:3419.
Long Name
Sphingomyelin Phosphodiesterase 1, Acid Lysosomal
Entrez Gene IDs
6609 (Human); 20597 (Mouse); 308909 (Rat)
Alternate Names
Acid sphingomyelinase; aSMase; ASMsphingomyelin phosphodiesterase; EC 3.1.4.12; NPD; SMPD1; sphingomyelin phosphodiesterase 1, acid lysosomal

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Citations for Human SMPD1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Differential regulation of microRNA-15a by radiation affects angiogenesis and tumor growth via modulation of acid sphingomyelinase
    Authors: S Rana, C Espinosa-D, R Ruhl, N Chatterjee, C Hudson, E Fraile-Bet, A Agarwal, S Khou, CR Thomas, S Anand
    Sci Rep, 2020-03-27;10(1):5581.
    Species: Mouse
    Sample Types: Cell Culture Lysates
    Applications: Western Blot
  2. Acid Sphingomyelinase regulates the localization and trafficking of palmitoylated proteins
    Authors: Xiahui Xiong, Chia-Fang Lee, Wenjing Li, Jiekai Yu, Linyu Zhu, Yongsoon Kim et al.
    Biology Open

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Reviews for Human SMPD1 Antibody

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Human SMPD1 Antibody
By Anonymous on 02/03/2017
Application: WB Sample Tested: 143B osteosarcoma cells Species: Human

30 micrograms of proteins per lane. Nitrocellulose membrane. 5% milk/PBS-Tween was used for blocking. Antibody dilution: 1:1000 in 1% milk/PBS-Tween. Lane 1=wild-type cells, lane 2 and 3 show two different knock-out clones obtained by CRISPR/Cas9 genome editing.
A loading control (GAPDH) is also shown.

Western Blot Human SMPD1 Antibody MAB5348