Human TLE2 Antibody

Catalog #: AF7216 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF7216
AF7216-SP

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Human TLE2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human TLE2 in direct ELISAs. In direct ELISAs, less than 2% cross-reactivity with recombinant human (rh) TLE1, rhTLE3, and rhTLE4 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human TLE2
Ile11-Ser193
Accession # Q04725

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Immunohistochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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TLE2 in Human Lung Mesothelioma. TLE2 was detected in immersion fixed paraffin-embedded sections of human lung mesothelioma using Sheep Anti-Human TLE2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7216) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TLE2

TLE2 (Transducin-Like Enhancer of Split 2; also ESG2 and Grg-2) is an 83-84 kDa member of the WD repeat Groucho/TLE family of transcriptional repressors. It is ubiquitously expressed, and is known to antagonize TCF (Wnt)-mediated signaling. TLE2 binds to other family members as a heterooligomer, or to itself as a homooligomer. While it possesses no intrinsic DNA-binding activity, it does modulate the activity of multiple factors such as FoxG1, Arx and histone H3. Human TLE2 is 743 amino acids (aa) in length. It contains a Gln-rich region that mediates oligomerization (aa 1-133), a CCN domain that contains an NLS (aa 195-256), and six WD repeats that mediate protein-protein interaction (aa 455-742). There are at least five potential phosphorylation sites that if used, may account for SDS-Page MWs exceeding 95 kDa. Potential isoform variants exist. One possesses a 21 aa substitution for aa 1-9, while a second shows the same substitution coupled to an additional 10 aa substitution for aa 683-743. A third isoform possesses an alternative start site at Met56 coupled to a deletion of aa 124-190. Over aa 11-193, human TLE2 shares 91% aa identity with mouse TLE2.

Long Name

Transducin-like Enhancer of Split 2

Entrez Gene IDs

7089 (Human); 21886 (Mouse); 299636 (Rat)

Alternate Names

enhancer of split groucho 2; ESG; ESG2; ESG2Enhancer of split groucho-like protein 2; FLJ41188; GRG2; TLE2; transducin-like enhancer of split 2 (E(sp1) homolog, Drosophila); transducin-like enhancer of split 2, homolog of Drosophila E(sp1); transducin-like enhancer protein 2