Human TRADD Antibody

Catalog #: MAB2658
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB2658
MAB2658-SP

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TRADD Antibody Summary

Species Reactivity

Human

Specificity

Detects human TRADD in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) BAFF R, rhTNF R1, rhHVEM, rhLT beta R, rhCD30, rhGITR, rh4-1BB, rhDR3, rhDR6, rhRANK, rhRELT, rhTAJ, rhEDAR, rhFas, recombinant mouse (rm) DC‑TRAIL R1, rmDC-TRAIL R2, rhTRAIL R3, rhTRAIL R4, rhNGF R, rhCD27, rhTNF RII, rhTWEAK R, rhTRAIL R2, or rhCD40 is observed.

Source

Monoclonal Mouse IgG_2A Clone # 313203

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human TRADD
Met1-Ala312
Accession # Q15628

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunocytochemistry

3-25 µg/mL

See below

Intracellular Staining by Flow Cytometry

0.25 µg/10⁶ cells

Human peripheral blood lymphocytes fixed with paraformaldehyde and permeabilized with saponin

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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TRADD in MCF‑7 Human Cell Line. TRADD was detected in immersion fixed MCF-7 human breast cancer cell line using Mouse Anti-Human TRADD Monoclonal Antibody (Catalog # MAB2658) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TRADD

TRADD (TNF R1A-associated death domain protein) is a 34 kDa protein that is expressed nearly ubiquitously. TRADD functions as an adaptor protein associating with the cytoplasmic domain of TNF receptor 1 or to another adaptor, FADD (Fas-interacting DD) to form the DISC (death inducing signaling complex). Human TRADD is an alpha-helical, Greek key, death domain-containing protein that is 77% identical to both mouse and rat TRADD.

Long Name

TNFRSF1A-associated via Death Domain

Entrez Gene IDs

8717 (Human)

Alternate Names

MGC11078; TNFRSF1A-associated via death domainTNFR1-associated DEATH domain protein; TRADD; tumor necrosis factor receptor type 1 associated death domain protein; tumor necrosis factor receptor type 1-associated DEATH domain protein; tumor necrosis factor receptor-1-associated protein

Product Datasheets

Product Datasheet

COA

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Related Research Areas

Citation for Human TRADD Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1