Human TRAF-4 Antibody

Catalog #: AF3279 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF3279
AF3279-SP

Detection of Human TRAF‑4 by Western Blot.

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All TRAF-4 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TRAF-4 Antibody Summary

Species Reactivity

Human

Specificity

Detects human TRAF-4 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human TRAF-1, -2, -3, -5, or -6 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human TRAF-4
Met1-Ser470
Accession # Q9BUZ4

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human TRAF‑4 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, Raji human Burkitt's lymphoma cell line, and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL Goat Anti-Human TRAF-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3279) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional reference, recombinant human TRAF-1, TRAF-2, TRAF-3, TRAF-4, TRAF-5, and TRAF-6 (2 ng/lane) were included. A specific band for TRAF-4 was detected at approximately 53 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TRAF-4

Tumor Necrosis Factor (TNF) Receptor-Associated Factors (TRAFs) are a family of adaptor proteins that interact with a wide range of cell surface receptors and participate in the regulation of cell survival, proliferation, differentiation, and stress response. TRAFs were identified by their ability to form complexes with TNF receptor superfamily members but more recently are reported to also bind to Toll/IL-1 receptor family members and mediate cellular signaling. Six members of the TRAF family have been identified. All TRAF proteins have a homologous C-terminal TRAF domain that can bind the cytoplasmic domain of receptors as well as other TRAFs. TRAFs 2-6 have N-terminal RING and zinc finger domains that are involved in signaling downstream events. TRAF-4, also known as CART1 and MLN62, is a 470 amino acid, 53 kDa protein. TRAF4 is unique member of the TRAF family that localizes to the nucleus and does not regulate signaling through cell surface receptors. TRAF-4 is highly expressed in human breast carcinomas and during embryogenisis.

Long Name

TNF Receptor-Associated Factor 4

Entrez Gene IDs

9618 (Human); 22032 (Mouse)

Alternate Names

CART1; CART1RING finger protein 83; Cysteine-rich domain associated with RING and Traf domains protein 1; Metastatic lymph node gene 62 protein; MLN 62; MLN62; MLN62cysteine-rich domain associated with ring and TRAF domain; RNF83malignant 62; TNF receptor-associated factor 4; TRAF4; TRAF-4; tumor necrosis receptor-associated factor 4A