Human TSPAN2 Antibody

Catalog #: MAB7876 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB7876
MAB7876-SP

Detection of TSPAN2 in NS0 Mouse Cell Line Co-transfected with TSPAN2 and eGFP by Flow Cytometry.

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Human TSPAN2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human TSPAN2 in ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 822509

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human TSPAN2
Met1-Ile221
Accession # O60636

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Flow Cytometry

2.5 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of TSPAN2 in NS0 Mouse Cell Line Co-transfected with TSPAN2 and eGFP by Flow Cytometry. NS0 mouse myeloma cell line co-transfected with human TSPAN2 and eGFP was stained with either (A) Mouse IgG_2BIsotype Control (Catalog # MAB0041) or (B) Mouse Anti-Human TSPAN2 Monoclonal Antibody (Catalog # MAB7876) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TSPAN2

TSPAN2 (tetraspanin-2) is a member of the transmembrane 4 superfamily. It is a cell surface 221 amino acid (aa) protein with 4 transmembrane segments and a large extracellular loop (aa 112‑188) that contains four conserved cysteines and one potential N-glycosylation site. Both the N- and C-termini are intracellular. In humans, TSPAN2 expressed sequence tags have been found in the pregnant uterus, T cell, and fetal heart. In rats, TSPAN2 is thought to be involved in oligodendrocyte differentiation. Human TSPAN2 shares 94% and 95% aa sequence identity with mouse and rat TSPAN2, respectively.

Long Name

Tetraspanin 2

Entrez Gene IDs

10100 (Human); 70747 (Mouse); 64521 (Rat)

Alternate Names

FLJ12082; NET3; new EST tetraspan 3; tetraspan 2; Tetraspan NET-3; tetraspan TM4SF; tetraspanin 2 isoform; tetraspanin 2; tetraspanin-2; TSN2; TSPAN2; TSPAN-2