Human TSPAN2 Antibody

Catalog #: MAB7876 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB7876-SP	Ships in 5-7 Days	25 ug / $149
MAB7876	In Stock	100 ug / $429

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Detection of TSPAN2 in NS0 Mouse Cell Line Co-transfected with TSPAN2 and eGFP by Flow Cytometry.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TSPAN2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human TSPAN2 in ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 822509

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human TSPAN2
Met1-Ile221
Accession # O60636

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Flow Cytometry

2.5 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of TSPAN2 in NS0 Mouse Cell Line Co-transfected with TSPAN2 and eGFP by Flow Cytometry. NS0 mouse myeloma cell line co-transfected with human TSPAN2 and eGFP was stained with either (A) Mouse IgG_2BIsotype Control (Catalog # MAB0041) or (B) Mouse Anti-Human TSPAN2 Monoclonal Antibody (Catalog # MAB7876) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TSPAN2

TSPAN2 (tetraspanin-2) is a member of the transmembrane 4 superfamily. It is a cell surface 221 amino acid (aa) protein with 4 transmembrane segments and a large extracellular loop (aa 112‑188) that contains four conserved cysteines and one potential N-glycosylation site. Both the N- and C-termini are intracellular. In humans, TSPAN2 expressed sequence tags have been found in the pregnant uterus, T cell, and fetal heart. In rats, TSPAN2 is thought to be involved in oligodendrocyte differentiation. Human TSPAN2 shares 94% and 95% aa sequence identity with mouse and rat TSPAN2, respectively.

Long Name

Tetraspanin 2

Entrez Gene IDs

10100 (Human); 70747 (Mouse); 64521 (Rat)

Alternate Names

FLJ12082; NET3; new EST tetraspan 3; tetraspan 2; Tetraspan NET-3; tetraspan TM4SF; tetraspanin 2 isoform; tetraspanin 2; tetraspanin-2; TSN2; TSPAN2; TSPAN-2