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Human Urocortin Antibody

Catalog #: MAB7447
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB7447
MAB7447-SP
Detection of Human Urocortin by Western Blot.
2 Images
Product Details
Citations (2)
FAQs
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Urocortin Antibody Summary

Species Reactivity
Human
Specificity
Detects human Urocortin in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG2B Clone # 749606
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Urocortin
Arg26-Lys124 (predicted)
Accession # P55089
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Immunohistochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Urocortin antibody by Western Blot. View Larger

Detection of Human Urocortin by Western Blot. Western blot shows lysates of SH-SY5Y human neuroblastoma cell line, HepG2 human hepatocellular carcinoma cell line, HeLa human cervical epithelial carcinoma cell line, and HEK293 human embryonic kidney cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human Urocortin Monoclonal Antibody (Catalog # MAB7447) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Urocortin at approximately 5 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Immunohistochemistry Urocortin antibody in Human Brain by Immunohistochemistry (IHC-P). View Larger

Urocortin in Human Brain. Urocortin was detected in immersion fixed paraffin-embedded sections of human brain using Mouse Anti-Human Urocortin Monoclonal Antibody (Catalog # MAB7447) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal processes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Urocortin

Urocortin (UCN, UCN1) is a member of the widely expressed corticotropin releasing factor (CRF) peptide family that includes UCN1-3 and CRF. UCN stimulates cAMP and MAPK pathways via either of two G-protein coupled receptors, CRFR1 and CRFR2, while other family members prefer CRFR1 (CRF) or CRFR2 (UCN2, UCN3). In the brain, UCN has been implicated in counteracting stress-provoked anxiety and suppressing appetite. It also modulates metabolic activity in peripheral tissues and protects the heart from ischemia/reprofusion injury. The UCN cDNA encodes 124 aa, including a 25 aa signal sequence, a 57 aa propeptide, and a 42 aa mature active 5 kDa UCN peptide. The proform of human UCN (aa 26-124, 11 kDa predicted) shares approximately 80% aa sequence identity with mouse and rat UCN.

Entrez Gene IDs
7349 (Human); 22226 (Mouse)
Alternate Names
MGC129974; MGC129975; UCN; UI; UROC; Urocortin; urocortin, preproprotein; UROCUI

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Citations for Human Urocortin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Dysregulation of bladder corticotropin-releasing hormone receptor in the pathogenesis of human interstitial cystitis/bladder pain syndrome
    Authors: JF Jhang, LA Birder, YH Jiang, YH Hsu, HC Ho, HC Kuo
    Sci Rep, 2019-12-16;9(1):19169.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  2. Urocortin suppresses endometrial cancer cell migration via CRFR2 and its system components are differentially modulated by estrogen
    Authors: GL Owens, KM Lawrence, TR Jackson, EJ Crosbie, BS Sayan, HC Kitchener, PA Townsend
    Cancer Med, 2017-01-21;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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