Human VGLUT1/SLC17A7 Antibody

Catalog #: MAB9054 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB9054
MAB9054-SP

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human VGLUT1/SLC17A7 Antibody Summary

Species Reactivity

Human

Specificity

Detects human VGLUT/SLC17A7 in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 732607

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E.coli-derived recombinant human VGLUT/SLC17A7
Asn93-Thr115
Accession # Q9P2U7

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunohistochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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VGLUT1/SLC17A7 in Human Brain. VGLUT1/SLC17A7 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Mouse Anti-Human VGLUT1/SLC17A7 Monoclonal Antibody (Catalog # MAB9054) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to synaptic vesicles. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VGLUT1/SLC17A7

Vesicular glutamate transporter 1 (VGLUT1), also known as SLC17A7, is an integral membrane protein involved in glutamate uptake into synaptic vesicles. The vesicular glutamate transporter (VGLUT) mediates the accumulation of neurotransmitter (glutamate) into synaptic vesicles. It is expressed in pre-synaptic vesicles. VGLUT proteins appear to be differentially expressed in myelinated afferents located in specific axonal populations within the spinal cord. VGLUT proteins are among the most specific markers for the identification of glutamatergic neurons. It is an essential molecular component of glutamate synaptic transmission, and its de-regulation can result synaptic failure and excitotoxicity.

Long Name

Vesicular Glutamate Transporter 1

Entrez Gene IDs

57030 (Human); 72961 (Mouse); 116638 (Rat)

Alternate Names

BNPI; BNPIbrain-specific Na-dependent inorganic phosphate cotransporter; Brain-specific Na(+)-dependent inorganic phosphate cotransporter; member 7; SLC17A7; solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter); Solute carrier family 17 member 7; VGLUT1; VGLUT1vesicular glutamate transporter 1