Human WASP Antibody

Name: Human WASP Antibody
Brand: R&D Systems
SKU: MAB3070
Rating: 4.5 (2 reviews)

Catalog #: MAB3070
1 Citation 2 Reviews Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB3070
MAB3070-SP

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Product Details

Citations (1)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human WASP Antibody Summary

Species Reactivity

Human

Specificity

Detects human WASP in direct ELISAs and Western blots.

Source

Monoclonal Mouse IgG₁ Clone # 336925

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human WASP
Lys230-Gln310
Accession # P42768

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human WASP

Immunocytochemistry

8-25 µg/mL

Immersion fixed U937 human histiocytic lymphoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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WASP in U937 Human Cell Line. WASP was detected in immersion fixed U937 human histiocytic lymphoma cell line using Mouse Anti-Human WASP Monoclonal Antibody (Catalog # MAB3070) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: WASP

WASP is a 54kDa protein belonging to the WASP/SCAR/WAVE family of proteins involved in actin polymerization. It is a highly conserved protein and is expressed selectively in hematopoietic stem cell-derived lineages. WASP contains multiple domains with unique functions. Mutations in WASP are associated with diseases of the hematopoietic/immunologic system.

Long Name

Wiskott-Aldrich Syndrome Protein

Entrez Gene IDs

7454 (Human); 22376 (Mouse)

Alternate Names

IMD2THC; Thrombocytopenia 1; WAS; WASP; WiskTHC1; wiskthrombocytopenia 1 (X-linked)

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human WASP Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Effect of ovarian steroids on gene expression profile in human uterine microvascular endothelial cells.
Authors: Yasuo T, Kitaya K
Fertil. Steril., 2008-08-09;92(2):709-21.
Species: Human
Sample Types: Cell Lysates, Whole Tissue
Applications: IHC-P, Western Blot