Human ZMIZ1/Zimp10 Antibody

Catalog #: AF8107 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF8107
AF8107-SP

Detection of Human ZMIZ1/Zimp10 by Western Blot.

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All ZMIZ1/Zimp10 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human ZMIZ1/Zimp10 Antibody Summary

Species Reactivity

Human

Specificity

Detects human ZMIZ1/Zimp10 in direct ELISAs and Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human ZMIZ1/Zimp10
Pro878-Ala981
Accession # Q9ULJ6

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunohistochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human ZMIZ1/Zimp10 by Western Blot. Western blot shows lysates of LNCaP human prostate cancer cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human ZMIZ1/Zimp10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8107) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ZMIZ1/Zimp10 at approximately 120-130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

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ZMIZ1/Zimp10 in Human Ovary. ZMIZ1/Zimp10 was detected in immersion fixed paraffin-embedded sections of human ovary using Sheep Anti-Human ZMIZ1/Zimp10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8107) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ZMIZ1/Zimp10

Human ZMIZ1 (zinc finger and Miz-domain containing 1), previously called Zimp10 (zinc finger, Miz1, PIAS-like protein on chromosome 10) is a 1067 amino acid (aa), approximately 130 kDa cytoplasmic and nuclear protein. It is a coactivator of the androgen receptor (AR), and enhances AR-mediated transcription and AR sumoylation. It also coactivates other transcription factors such as SMAD4 and p53. It is normally expressed in human ovary, testis and prostate epithelial cells. The ZIMZ1 gene can be fused to ABL1 in a B-cell acute lymphocytic leukemia. Within the region used as an immunogen, human ZMIZ1 shares 98% and 97% aa sequence identity with mouse and rat ZMIZ1, respectively. A 997 aa isoform with an alternate sequence replacing aa 1-94 has been described.

Long Name

Zinc Finger, MIZ-Type Containing 1

Entrez Gene IDs

57178 (Human)

Alternate Names

FLJ13541; hZIMP10; KIAA1224; KIAA1224Zimp10; MIZ; PIAS-like protein Zimp10; RAI17; RAI17TRAFIP10; retinoic acid induced 17; Retinoic acid-induced protein 17; RP11-519K18.1; TRAFIP10; Zimp10; ZIMP10FLJ00092 protein; zinc finger MIZ domain-containing protein 1; zinc finger, MIZ-type containing 1; zinc finger-containing, Miz1, PIAS-like protein on chromosome 10; ZMIZ1