ZNF206 (zinc finger protein 206; also ZFP206) is an 80 kDa SCAN-zinc finger transcription factor that is active in embryonic stem cells and appears to positively regulate transcriptional regulators that maintain stem cell pluripotency. Human ZNF206 is 725 amino acids in length. It contains one SCAN box (aa 1‑71) that mediates oligomerization, followed by a proline-rich region (aa 104‑175) and 14 consecutive C2H2-type zinc fingers (aa 292‑719). Mouse ZNF206 apparently has multiple isoforms, none of which are well characterized. The human gene may also show alternate splicing. Human ZNF206 is 72% aa identical to mouse ZNF206.
Human ZNF206 Antibody
R&D Systems | Catalog # AF4020
Key Product Details
Species Reactivity
Human
Applications
Western Blot, Immunocytochemistry
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
E. coli-derived recombinant human ZNF206
Met1-Val283
Accession # Q96SZ4
Met1-Val283
Accession # Q96SZ4
Specificity
Detects human ZNF206 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human ZNF24 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human ZNF206 Antibody
ZNF206 in BG01V Human Embryonic Stem Cells.
ZNF206 was detected in immersion fixed BG01V human embryonic stem cells using Goat Anti-Human ZNF206 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4020) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with tubulin (green). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
Applications for Human ZNF206 Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed undifferentiated BG01V human embryonic stem cells
Sample: Immersion fixed undifferentiated BG01V human embryonic stem cells
Western Blot
0.1 µg/mL
Sample: Recombinant Human ZNF206
Sample: Recombinant Human ZNF206
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: ZNF206
Long Name
Zinc Finger Protein 206/Zinc Finger and SCAN Domain Containing 10
Alternate Names
Zfp206, ZSCAN10
Gene Symbol
ZSCAN10
UniProt
Additional ZNF206 Products
Product Documents for Human ZNF206 Antibody
Product Specific Notices for Human ZNF206 Antibody
For research use only
Citations for Human ZNF206 Antibody
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Detection & Visualization of Antibody Binding
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars