Mouse Afamin Antibody

Catalog #: AF8119 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF8119
AF8119-SP

Detection of Mouse Afamin by Western Blot.

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Mouse Afamin Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Afamin in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human Afamin is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Afamin
Leu22-Met608
Accession # O89020

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Mouse Afamin by Western Blot. Western blot shows mouse serum. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Mouse Afamin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8119) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Afamin at approximately 87 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Afamin

Afamin, also known as AFM or alpha -Albumin, is an 84-88 kDa secreted monomeric glycoprotein member of the Alb/albumin family of molecules. Expressed by hepatocytes, CNS endothelial cells and osteoclasts, Afamin circulates in the blood at low μg/mL concentrations. Afamin is known to bind and transport vitamin E, particularly under conditions where lipoprotein is limited. This is likely to be important in follicular fluid and CSF. Afamin also serves as an osteoclast-derived chemoattractant for preosteoblasts, providing a rationale for the observation that bone formation often follows bone resorption. Mature mouse Afamin is 587 amino acids (aa) in length (aa 22-608). It contains three consecutive albumin domains (aa 22-210, 211-403 and 404-599) that contain characteristic 5 or 6 intrachain disulfide bonds. Full-length mouse Afamin shares 67% aa sequence identity with human Afamin.

Entrez Gene IDs

173 (Human); 280662 (Mouse); 282708 (Rat)

Alternate Names

Afamin; AFM; ALB2; ALB2alpha-Alb; ALBA; ALBAalpha-albumin; ALF; Alpha-Alb; Alpha-albumin; MGC125338; MGC125339