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Mouse CD117/c-kit PE-conjugated Antibody

Catalog #: FAB1356P
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB1356P-100
FAB1356P-025
Detection of CD117/c-kit in Mouse Splenoctyes by Flow Cytometry.
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Product Details
Citations (2)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CD117/c-kit PE-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse SCF R in direct ELISAs.
Source
Monoclonal Rat IgG2A Clone # 180627
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse SCF R
Gln25-Thr519 (Ala207Glu)
Accession # P05532
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CD117/c-kit antibody in Mouse Splenoctyes antibody by Flow Cytometry. View Larger

Detection of CD117/c-kit in Mouse Splenoctyes by Flow Cytometry. Mouse splenocytes gated on hematopoietic lineage negative cells were stained with Rat Anti-Mouse CD117/c-kit PE-conjugated Monoclonal Antibody (Catalog # FAB1356P) and Rat Anti-Mouse Sca-1/Ly6 APC-conjugated Monoclonal Antibody (Catalog # FAB1226A). Quadrant markers were set based on control antibody staining (Catalog # IC006P). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: CD117/c-kit

SCF R, also known as c-kit proto-oncogene protein and CD117, is a 145-160 kDa member of the type III tyrosine kinase family of proteins (1,2). It is expressed on hematopoietic and endothelial cell progenitors, mast cells, germ cells, melanocytes, trigeminal neurons, glial cells, placenta, kidney, lung, and gut. SCF R is a type I transmembrane glycoprotein that undergoes splicing during spermatogenesis. Two isoforms of 50 kDa and 30 kDa are generated that represent C-terminal intracellular functional products (3). SCF R also undergoes cleavage by select cell types, generating a 95-100 kDa soluble form (4). Over amino acids 25-519, mouse SCF R shares 73% and 87% amino acid sequence identity with human and rat SCF R, respectively.

References
  1. Heldin, C-H. and J. Lennartsson (2013) Cold Spring Harb. Perspect. Biol. 5:a009100.
  2. Lennartsson, J.  and L. Ronnstrand (2012) Physil. Rev. 92:1619.
  3. Zhang, L. et al. (2013) BMC Dev. Biol. 13:38.
  4. Broudy, V.C. et al. (2001) Cytokine 15:188.
Long Name
Stem Cell Factor Receptor
Entrez Gene IDs
3815 (Human); 16590 (Mouse); 64030 (Rat)
Alternate Names
CD117 antigen; CD117; ckit; c-kit; EC 2.7.10; EC 2.7.10.1; KIT; PBT; piebald trait; Proto-oncogene c-Kit; proto-oncogene tyrosine-protein kinase Kit; SCF R; SCFR; SCFRmast/stem cell growth factor receptor; soluble KIT variant 1; Tyrosine-protein kinase Kit; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein

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Citations for Mouse CD117/c-kit PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. IL-21 Reduces Immediate Hypersensitivity Reactions in Mouse Skin by Suppressing Mast Cell Activation or IgE Production.
    Authors: Tamagawa-Mineoka R, Kishida T, Mazda O
    J. Invest. Dermatol., 2011-03-24;131(7):1513-20.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Mobilized bone marrow progenitor cells serve as donors of cytoprotective genes for cardiac repair.
    Authors: Dai Y, Ashraf M, Zuo S, Uemura R, Dai YS, Wang Y, Haider HKh, Li T, Xu M
    J. Mol. Cell. Cardiol., 2007-12-07;44(3):607-17.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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