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Mouse CD5 Biotinylated Antibody

Catalog #: BAM115
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BAM115
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Citations (1)
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Mouse CD5 Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CD5 in ELISAs.
Source
Monoclonal Rat IgG2A Clone # 53-7.3
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse thymus and spleen cells
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
Mouse splenocytes
Immunohistochemistry
8-25 µg/mL
Immunoprecipitation
Ledbetter, J.A. and L.A. Herzenberg (1979) Immunol. Rev. 47:63.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD5

CD5, also known as Leu-1, is a glycoprotein expressed on thymocytes, T cells, and the B1a subpopulation of B cells. CD5 is a member of the scavenger receptor cysteine-rich protein superfamily and the ligand of the C-type lectin, CD72. It modulates signaling through the antigen receptors on T and B cells.

Entrez Gene IDs
921 (Human); 12507 (Mouse)
Alternate Names
CD5 antigen (p56-62); CD5 antigen; CD5 molecule; CD5; LEU1T-cell surface glycoprotein CD5; Lymphocyte antigen T1/Leu-1; T1

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Citation for Mouse CD5 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Natural IgM is required for suppression of inflammatory arthritis by apoptotic cells.
    Authors: Notley CA, Brown MA, Wright GP, Ehrenstein MR
    J. Immunol., 2011-03-07;186(8):4967-72.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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