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Mouse CD5 Fluorescein-conjugated Antibody

Catalog #: FAB115F Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB115F
Detection of CD5 in Mouse Splenocytes by Flow Cytometry.
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Mouse CD5 Fluorescein-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CD5 in flow cytometry.
Source
Monoclonal Rat IgG2A Clone # 53-7.3
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse thymus or spleen
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CD5 antibody in Mouse Splenocytes antibody by Flow Cytometry. View Larger

Detection of CD5 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD3 APC-conjugated Monoclonal Antibody (Catalog # FAB4841A) and either (A) Rat Anti-Mouse CD5 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB115F) or (B) Rat IgG2AFluorescein Isotype Control (Catalog # IC006F). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: CD5

Mouse CD5 has been shown to react with a mouse monomorphic CD5 (Ly-1), a member of scavenger receptor cysteine-rich protein superfamily (1). CD5 is a membrane glycoprotein found on thymocytes, T cells, and a subset of B cells but not on NK cells (2‑6). It is a ligand of the B cell differentiation antigen CD72.

References
  1. Ledbetter, J.A. and L.A. Herzenberg, (1979) Immunol. Rev. 47:63.
  2. Ledbetter, J.A. et al. (1980) J. Exp. Med. 152:280.
  3. van Ewijk, W. et al. (1981) J. Immunol. 127:2594.
  4. Hayakawa, K. et al. (1983) J. Exp. Med. 157:202.
  5. Luo, W. et al. (1992) J. Immunol. 148:1630.
  6. Lanier, L.L. et al. (1986) J. Immunol. 137:2735.
Entrez Gene IDs
921 (Human); 12507 (Mouse)
Alternate Names
CD5 antigen (p56-62); CD5 antigen; CD5 molecule; CD5; LEU1T-cell surface glycoprotein CD5; Lymphocyte antigen T1/Leu-1; T1

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