Mouse CD5 Antibody

Catalog #: MAB115 Datasheet / COA / SDS

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MAB115
MAB115-SP

All CD5 Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CD5 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse CD5.

Source

Monoclonal Rat IgG_2A Clone # 53-7.3

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse thymus or spleen

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

Mouse splenocytes

Immunohistochemistry

van Ewijk, W. et al. (1981) J. Immunol. 127:2594.

Immunoprecipitation

Ledbetter, J.A. and L.A. Herzenberg (1979) Immunol. Rev. 47:63.

Blocking B Cell Proliferation

Bikah, G. et al. (1998) Int. Immunol. 10:1185.

Complement-dependent Cytotoxicity

Ledbetter, J.A. et al. (1980) J. Exp. Med. 152:280.

CyTOF-reported

Spitzer, M. et al. (2015) Science 349: 1259425. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD5

Mouse CD5 has been shown to react with a mouse monomorphic CD5 (Ly-1), a Group B member of the scavenger receptor cysteine-rich protein superfamily (1). CD5 is a 67 kDa type I transmembrane glycoprotein found on thymocytes, T cells, and a subset of B cells but not on NK cells (2‑6). It is likely a ligand of the B cell differentiation antigen CD72 and is known to associate with CD6 (1). Mouse and human extracellular domains share 55% amino acid sequence indentity.

References