Mouse MMP-9 Biotinylated Antibody Summary
Ala20-Pro730
Accession # P41245
Customers also Viewed
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
View Larger
MMP‑9 in Mouse Splenocytes. MMP-9 was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse MMP-9 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF909) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
View Larger
MMP‑9 in Mouse Thymus. MMP‑9 was detected in immersion fixed paraffin-embedded sections of mouse thymus using Goat Anti-Mouse MMP‑9 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF909) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in thymocytes.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MMP-9
Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-9 (gelatinase B) can degrade a broad range of substrates including gelatin, collagen types IV and V, elastin, and proteoglycan core protein. It is believed to act synergistically with interstitial collagenase (MMP-1) in the degradation of fibrillar collagens as it degrades their denatured gelatin forms. MMP-9 is produced by keratinocytes, monocytes, macrophages, and PMN leukocytes. MMP-9 is present in most cases of inflammatory responses. Structurally, MMP-9 may be divided into five distinct domains: a pro-domain which is cleaved upon activation, a gelatin-binding domain consisting of three contiguous fibronectin type II units, a catalytic domain containing the zinc binding site, a proline-rich linker region, and a carboxyl terminal hemopexin-like domain. Compared to the human MMP-9
(Catalog # 911-MP), the mouse enzyme contains extra sequences in the linker region and in the hemopexin-like domain, respectively.
Product Datasheets
Citations for Mouse MMP-9 Biotinylated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
2
Citations: Showing 1 - 2
Filter your results:
Filter by:
-
Inhibition of the mTOR pathway in abdominal aortic aneurysm: implications of smooth muscle cell contractile phenotype, inflammation, and aneurysm expansion
Authors: Guangxin Li, Lingfeng Qin, Lei Wang, Xuan Li, Alexander W. Caulk, Jian Zhang et al.
American Journal of Physiology-Heart and Circulatory Physiology
-
Attenuation of myocardial injury in mice with functional deletion of the circadian rhythm gene mPer2.
Authors: Virag JA, Dries JL, Easton PR, Friesland AM, DeAntonio JH, Chintalgattu V, Cozzi E, Lehmann BD, Ding JM, Lust RM
Am. J. Physiol. Heart Circ. Physiol., 2010-01-08;298(3):H1088-95.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsIsotype Controls
Reconstitution Buffers
Staining Reagents
Supplemental Cell Selection Products
Supplemental ELISA Products
Reviews for Mouse MMP-9 Biotinylated Antibody
There are currently no reviews for this product. Be the first to review Mouse MMP-9 Biotinylated Antibody and earn rewards!
Have you used Mouse MMP-9 Biotinylated Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
