Mouse NELL2 Antibody

Catalog #: AF6736 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF6736
AF6736-SP

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Mouse NELL2 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse NELL2 in direct ELISAs. In direct ELISAs, approximately 15% cross-reactivity with recombinant human (rh) NELL2 and less than 3% cross-reactivity with rhNELL1 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse NELL2
Leu25-Leu819
Accession # NP_058023

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Immunohistochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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NELL2 in Mouse Spinal Cord. NELL2 was detected in perfusion fixed frozen sections of mouse spinal cord using Sheep Anti-Mouse NELL2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6736) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to motoneurons. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NELL2

NELL2 (neural EGF-like like protein 2) is a secreted, 130-140 kDa glycoprotein member of the EGF-like domain containing family, Laminin G/N-TSP1/Pentraxin gene superfamily of molecules. It is expressed in both fetal and postnatal neurons, being found in pyramidal and hypothalamic glutamatergic neurons, and in GABAergic cerebellar Purkinje cells. It has multiple functions, including the induction of GnRH release and the promotion of neuronal neurite extension and synapse formation. The mouse NELL2 precursor is 819 amino acids (aa) in length. It contains a 27 aa signal sequence plus a 792 aa mature region. The mature region possesses an N‑terminal TSP domain (aa 33-261), two VWFC domains (aa 275-399), six consecutive EGF-like domains (aa 400-640), and three C-terminal VWFC domains (aa 641‑816). Secreted NELL2 forms 400 kDa noncovalent homotrimers. There are four potential alternate splice forms. One utilizes an alternate start site at Met4, while a second shows a 16 aa substitution for aa 400-436. Based on rat, two other isoforms are likely to exist. A third isoform (cNELL2) should generate a 90 kDa phosphorylated cytosolic molecule that possesses a deletion of aa 22-64, while a fourth, 30 kDa isoform (NELL2-Tsp) should arise due to a premature truncation after Ala261. This last isoform is soluble and predicted to trimerize with full-length NELL2, yielding lower MW complexes. Over aa 24-819, mouse NELL2 shares 97% and 94% aa identity with rat and human NELL2, respectively.

Long Name

NEL-like 2

Entrez Gene IDs

4753 (Human); 54003 (Mouse); 81734 (Rat)

Alternate Names

Mel91; nel (chicken)-like 2; NELL2; NEL-like 2 (chicken); NEL-like protein 2; Nel-related protein 2; NRP2; NRP2neural epidermal growth factor-like 2; protein kinase C-binding protein NELL2