Mouse/Rat alpha 1B-Glycoprotein Antibody

Catalog #: AF8537
1 Citation Datasheet / COA / SDS

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AF8537

Detection of Mouse and Rat alpha 1B-Glycoprotein by Western Blot.

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Citations (1)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse/Rat alpha 1B-Glycoprotein Antibody Summary

Species Reactivity

Mouse, Rat

Specificity

Detects mouse and rat alpha 1B-Glycoprotein in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human alpha 1B-Glycoprotein observed.

Source

Polyclonal Rabbit IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse alpha 1B-Glycoprotein
Leu21-Gly512
Accession # Q19LI2

Formulation

Supplied as a solution in PBS containing BSA, Glycerol and Sodium Azide.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1:1000 dilution

See below

Simple Western

1:100 dilution

See below

Immunocytochemistry

1:20-1:50 dilution

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Mouse and Rat alpha 1B-Glycoprotein by Western Blot. Western blot shows mouse plasma and rat plasma. PVDF membrane was probed with 1:1000 dilution of Rabbit Anti-Mouse/Rat a 1B-Glycoprotein Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8537) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for a 1B-Glycoprotein at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry

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alpha 1B-Glycoprotein in Hepa 1‑6 Mouse Cell Line. a 1B-Glycoprotein was detected in immersion fixed Hepa 1-6 mouse hepatoma cell line using Rabbit Anti-Mouse/Rat a 1B-Glycoprotein Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8537) at 1:20 dilution for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Simple Western

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Detection of Mouse and rat alpha 1B-Glycoprotein by Simple Western^TM. Simple Western lane view shows lysates mouse plasma and rat plasma, loaded at 0.2 mg/mL. A specific band was detected for alpha 1B-Glycoprotein at approximately 113 kDa (as indicated) using 1:100 dilution of Rabbit Anti-Mouse/Rat alpha 1B-Glycoprotein Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8537). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C, as supplied.
1 month, 2 to 8 °C under sterile conditions after opening.
6 months, -20 to -70 °C under sterile conditions after opening.

Background: alpha 1B-Glycoprotein

The alpha 1B-Glycoprotein is a plasma glycoprotein, encoded by the A1BG gene with an unknown function. The protein shows sequence similarity to the variable regions of some immunoglobulin supergene family member proteins.

Entrez Gene IDs

1 (Human); 117586 (Mouse)

Alternate Names

A1B; A1BG; ABG; alpha 1BGlycoprotein; alpha 1B-Glycoprotein; Alpha-1-B glycoprotein;alpha1B-glycoprotein;Alpha-1B-glycoprotein;alpha-1-B-glycoprotein;DKFZp686F0970;epididymis secretory sperm binding protein Li 163pA;HEL-S-163pA; GAB

Product Datasheets

Product Datasheet

COA

SDS

Product Specific Notices

* Contains <0.1% Sodium Azide, which is not hazardous at this concentration according to GHS classifications. Refer to SDS for additional information and handling instructions.

Related Research Areas

Citation for Mouse/Rat alpha 1B-Glycoprotein Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1