Mouse SLAM/CD150 Antibody

Catalog #: AF4330 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4330
AF4330-SP

Detection of SLAM/CD150 in Mouse Splenocytes by Flow Cytometry.

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All SLAM/CD150 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse SLAM/CD150 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse SLAM/CD150 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human SLAM is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse SLAM/CD150
Thr25-Pro242
Accession # Q9QUM4

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse SLAM/CD150 (Catalog # 4330-SL)

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of SLAM/CD150 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were treated for 2 days with 50 ng/mL PMA and 500 ng/mL Ca²⁺ionomycin then stained with Sheep Anti-Mouse SLAM/CD150 Antigen Affinity-purified Poly-clonal Antibody (Catalog # AF4330, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by NorthernLights™ 637-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL011).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SLAM/CD150

The type I transmembrane glycoprotein Signaling Lymphocytic Activation Molecule (SLAM), also known as CD150, is the prototypic member of the SLAM subgroup of the CD2 protein family. CD2 family proteins function as adhesion molecules and modulators of the immune response (1). Mouse SLAM consists of a 218 amino acid (aa) extracellular domain (ECD) with two Ig-like domains, a 23 aa transmembrane segment, and a 78 aa cytoplasmic domain with three immunoreceptor tyrosine switch motifs (ITSM) (2). Alternate splicing generates an isoform with a substituted cytoplasmic domain (2). Within the ECD, mouse SLAM shares 58% and 83% aa sequence identity with human and rat SLAM, respectively. It is expressed as a 75 kDa molecule of which approximately 30 kDa is N-linked carbohydrate (2). SLAM is expressed on T cells, B cells, thymocytes, macrophages, dendritic cells, platelets, and hematopoietic stem cells (2‑7). It is upregulated on activated B cells and CD4⁺ and CD8⁺T cells, although it is downregulated on Th2 polarized cells (2, 3, 8). SLAM interacts homophilically with low affinity, and this interaction induces a Th0/Th1 response characterized by clonal expansion, production of IFN-gamma, and increased cytolytic activity of CD8⁺ T cells (2, 3, 9‑11). SLAM ligation also promotes B cell activation, allergen-induced eosinophil and mast cell activation, and macrophage responsiveness to LPS (4, 8, 12). In humans, SLAM functions as a cellular entry receptor for measles virus (13, 14).

References

Ma, C.S. et al. (2007) Annu. Rev. Immunol. 25:337.
Castro, A.G. et al. (1999) J. Immunol. 163:5860.
Cocks, B.G. et al. (1995) Nature 376:260.
Wang, N. et al. (2004) J. Exp. Med. 199:1255.
Hahm, B. et al. (2004) Virology 323:292.
Nanda, N. et al. (2005) Blood 106:3028.
Kiel, M.J. et al. (2005) Cell 121:1109.
Punnonen, J. et al. (1997) J. Exp. Med. 185:993.
Mavaddat, N. et al. (2000) J. Biol. Chem. 275:28100.
Aversa, G. et al. (1997) J. Immunol. 158:4036.
Mehrle, S. et al. (2008) Mol. Immunol. 45:796.
Wang, N. et al. (2006) Am. J. Respir. Cell Mol. Biol. 35:206.
Tatsuo, H. et al. (2000) Nature 406:893.
Hsu, E.C. et al. (2001) Virology 279:9.

Long Name

Signaling Lymphocytic Activation Molecule

Entrez Gene IDs

6504 (Human); 27218 (Mouse); 102135470 (Cynomolgus Monkey)

Alternate Names

CD 150; CD150; CD150IPO-3; CDw150; IPO-3; signaling lymphocytic activation molecule family member 1; signaling lymphocytic activation molecule; SLAM; SLAMCD150 antigen; SLAMF1