Simple Plex Human TIMP-2 Cartridge

Catalog #: SPCKB-PS-006178 Datasheet / COA / SDS

Simple Plex Human TIMP-2 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer

Catalog #	Availability	Size / Price	Qty
SPCKB-PS-006178
SPCKA-PS-006178
SPCKC-PS-006178

Control Products Available

896947: Simple Plex Control for Human TIMP-2

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Summary Precision Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Human TIMP-2 Cartridge Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Saliva (10 uL), Urine (10 uL)

Sensitivity

4.8 pg/mL

Assay Range

20.5 - 12,500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine)

Cross-reactivity

Cross-reactivity observed with 1 or more available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

Interference observed with 1 or more available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple plex assay for the detection of human Tissue Inhibitor of Metalloproteinases 2 (TIMP-2) in cell culture supernatant (CCS), serum, plasma (EDTA/Heparin), saliva, and urine.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	22	22
Mean (pg/mL)	183	9932	167	10585
Standard Deviation	8.3	587	11.2	991
CV%	4.5	5.9	6.7	9.4

Linearity

Sample containing and/or spiked with high concentrations for human TIMP-2 were serially diluted with Sample Diluent to produce sample within the dynamic range of the assay.

Scientific Data

N/A

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Human TIMP-2 Ella Assay Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: TIMP-2

TIMPs-1 through -4 regulate the activity of zinc metalloproteases known as MMPs, ADAMs and ADAMTSs. Structurally, TIMPs contain two domains. The N-terminal domain binds to the active site of mature metalloproteases via a 1:1 non-covalent interaction, blocking access of substrates to the catalytic site. In addition, The C-terminal domain of TIMP-1 and TIMP-2 binds to the hemopexin- like domain of pro-MMP-9 and pro-MMP-2, respectively. The latter binding is essential for the cell surface activation of MMP-2 by MMP-14.

Long Name:

Tissue Inhibitors of Metalloproteinases 2

Entrez Gene IDs:

7077 (Human); 21858 (Mouse); 29543 (Rat)

Alternate Names:

CSC-21Ktissue inhibitor of metalloproteinase 2; metalloproteinase inhibitor 2; TIMP metallopeptidase inhibitor 2; TIMP2; TIMP-2; Tissue inhibitor of metalloproteinases 2