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Synapsin I Pan Specific Antibody

Catalog #: PPS035
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PPS035
Detection of Synapsin I by Western Blot.
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Citations (1)
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Synapsin I Pan Specific Antibody Summary

Species Reactivity
Human, Mouse, Rat, Bovine
Specificity
This antibody is specific for the ~78 kDa Synapsin I doublet in Western blots of rat brain lysates. Immunolabeling is blocked by preadsorption of the antibody with the native protein immunogen.
Source
Polyclonal Rabbit IgG
Purification
Antigen Affinity-purified
Immunogen
Synapsin I
Formulation
10 μg per vial; lyophilized in 5 mM ammonium bicarbonate.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1:1000 dilution
See below
Immunohistochemistry
1:2000 dilution
Immunoprecipitation
0.5 µg/100 µg cell lysate
Immunofluorescence
1:2000 dilution

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Synapsin I antibody by Western Blot. View Larger

Detection of Synapsin I by Western Blot. Western blot of 10 μg of rat hippocampus lysate showing specific immunolabeling of the ~78 kDa Synapsin I doublet.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
This antibody should be reconstituted in 50 μL phosphate buffered saline (137 mM NaCl, 7.5 mM Na2HPO4, 2.7 mM KCl, 1.5 mM KH2PO4) before use.
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Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
The lyophilized product is stable at  ≤ ‑20° C for at least one year. After reconstitution the antibody should be stored at  ≤ ‑20° C.

Background: Synapsin I

Synapsin I is an 85 kDa neuron-specific, O-GlcNAcylated phosphoprotein that interacts with synaptic vesicles and regulates neurotransmitter release. It is concentrated in pre‑synaptic terminals where it anchors synaptic vesicles to the underlying cytoskeleton. Phosphorylation at various sites reduces Synapsin I binding to neurosecretory vesicles, making them available for neurotransmitter release. Rat Synapsin I is 704 amino acids (aa) in length and contains five domains, named A-E/F. Domains A‑D lie between amino acids 1-655, while E and F are alternate splice forms. Domain A (28 aa) regulates neurotransmitter release in a phosphorylation-dependent manner. Domains C (328 aa) and E (49 aa) anchor to actin in the synaptic region. Domains B (84 aa) and D (235 aa) promote actin and secretory vesicle interaction, and actin nucleation. Phosphorylation of S603 and S566 in domain D, and S9 in domain A, reduces the affinity of Synapsin I for actin and secretory vesicles.

References
  1. Yamagata, Y. (2003) J. Pharmacol. Sci. 93:22.
  2. Sakurada, K. et al. (2002) J. Biol. Chem. 277:45473.
  3. Hilfiker, S. et al. (2005) J. Neurosci. 25:2658.
  4. McCaffery, C.A. and L.J. DeGennaro (1986) EMBO J. 5:3167.
  5. Cole, R.N. and G.W. Hart (1999) J. Neurochem. 73:418.
Entrez Gene IDs
6853 (Human); 20964 (Mouse); 24949 (Rat)
Alternate Names
SYN1; SYN1a; SYN1b; Synapsin 1; Synapsin I; synapsin IBrain protein 4.1; synapsin-1; SYNI

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Citation for Synapsin I Pan Specific Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Neural differentiation medium for human pluripotent stem cells to model physiological glucose levels in human brain
    Authors: ME Mor, A Harvey, M Familari, M St Clair-G, S Viventi, RU de Iongh, FJ Cameron, M Dottori
    Brain research bulletin, 2021-05-19;173(0):141-149.
    Species: Human
    Sample Types: Protein
    Applications: Reverse Phase Protein Array

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