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Viral CMV UL146/vCXC1 Antibody

Catalog #: MAB620 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB620
MAB620-SP
Chemotaxis Induced by CMV UL146/vCXC1 and Neutralization by CMV UL146/vCXC1 Antibody.
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Viral CMV UL146/vCXC1 Antibody Summary

Species Reactivity
Viral
Specificity
Detects viral CMV UL146/vCXC1 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human CXCL1, 2, 3, 5, 6, 7, 8, 9, 10, 11, 12, 13, recombinant mouse CXCL1, 2, 6, 9, 10, 12, recombinant rat CXCL1, 3, recombinant viral MIP-I, -II, or -III is observed.
Source
Monoclonal Mouse IgG2A Clone # 76520
Purification
Protein A or G purified from ascites
Immunogen
E. coli-derived recombinant viral CMV UL146/vCXC1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Viral CMV UL146/vCXC1 (Catalog # 620-CM)
Neutralization
Measured by its ability to neutralize CMV UL146/vCXC1-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2. The Neutralization Dose (ND50) is typically 1.5-7.5 µg/mL in the presence of 0.3 µg/mL Recombinant Viral CMV UL146/vCXC1.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Chemotaxis Induced by CMV UL146/vCXC1 and Neutralization by CMV UL146/vCXC1 Antibody. View Larger

Chemotaxis Induced by CMV UL146/vCXC1 and Neutralization by CMV UL146/vCXC1 Antibody. Recombinant Viral CMV UL146/vCXC1 (Catalog # 620-CM) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Viral CMV UL146/vCXC1 (0.3 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Viral CMV UL146/vCXC1 Monoclonal Antibody (Catalog # MAB620). The ND50 is typically 1.5-7.5 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: UL146/vCXC1

Cytomegalovirus (CMV), a member of the beta herpesvirus subfamily, typically causes subclinical or latent infections in the normal adult population. However, CMV can cause congenital disease during pregnancy and is a human opportunistic pathogen that affects immunocompromised individuals. The CMV genome has been shown to contain homologs of cellular immunomodulatory proteins, including US28 (a CC chemokine receptor) and a MHC class I homolog. Virulent CMV clinical isolates have also been shown to carry at least 19 genes, designated UL133-UL151, that are not found in laboratory strains that have lost virulence characteristics. Two of these genes, UL146 and UL147, exhibit sequence similarity to CXC chemokines.

The CMV UL146 open-reading frame encodes a 117 amino acid residue precursor protein with a predicted 22 residues signal peptide that is cleaved to generate the mature protein. Recombinant UL146 has been shown to induce calcium mobilization, chemotaxis and degranulation of neutrophils.

References
  1. Dairaghi, D. et al. (1998) Sem. Virol. 8:377.
  2. Cha, T.A. et al. (1996) J. Virol.70:78.
Long Name
Cytomegalovirus UL146
Alternate Names
HHV5wtgp117; UL146; vCXC1; vCXCL1; Y18

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