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cAMP Antibody

Catalog #: MAB2146
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB2146
MAB2146-SP
cAMP in Human PBMCs.
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Product Details
Citations (3)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

cAMP Antibody Summary

Specificity
Detects cAMP. In a competition binding assay, this antibody does not cross-react with ATP, CTP, GTP, AMP, GMP, cGMP, or cUMP.
Source
Monoclonal Mouse IgG1 Clone # 250532
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Thryoglobulin-coupled cAMP
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry cAMP antibody in Human PBMCs by Immunocytochemistry (ICC). View Larger

cAMP in Human PBMCs. cAMP was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using 10 µg/mL Mouse Anti-cAMP Monoclonal Antibody (Catalog # MAB2146) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: cAMP

cAMP is a cyclic nucleotide generated from ATP by the activity of adenylate cyclase. It is a ubiquitous cytoplasmic second messenger important in many signal transduction pathways.

Long Name
Cyclic AMP
Alternate Names
cAMP; Cyclic AMP

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Citations for cAMP Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Adenosine-A2A Receptor Signaling Plays a Crucial Role in Sudden Unexpected Death in Epilepsy
    Authors: Hai-Ying Shen, Sadie B. Baer, Raey Gesese, John M. Cook, Landen Weltha, Shayla Q. Coffman et al.
    Frontiers in Pharmacology
  2. microRNA-208a in an early stage myocardial infarction rat model and the effect on cAMP-PKA signaling pathway
    Authors: Gao Feng
    Mol Med Rep, 2016-06-14;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Amyloid beta (Abeta) peptide directly activates amylin-3 receptor subtype by triggering multiple intracellular signaling pathways.
    Authors: Fu W, Ruangkittisakul A, MacTavish D, Shi JY, Ballanyi K, Jhamandas JH
    J. Biol. Chem., 2012-04-12;287(22):18820-30.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC, Western Blot

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