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FITC Antibody

Catalog #: MAB8980
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB8980
MAB8980-SP
Product Details
Citations (1)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

FITC Antibody Summary

Specificity
Detects Fluorescein Isothiocyanate (FITC) and Carboxyfluorescein (CFS) in direct ELISAs. In direct ELISAs, no cross-reactivity with Alexa Fluor® 488, Alexa Fluor® 594, Alexa Fluor® 647, Alexa Fluor® 700, Alexa Fluor® 750, NorthernLights™ NL493, NorthernLights™ 557, and NorthernLights™ 637 is observed.
Source
Monoclonal Mouse IgG Clone # 931845
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Fluorescein Isothiocyanate (FITC)
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunocytochemistry
8-25 µg/mL
Detects FITC-conjugated and CFS-conjugated antibodies in immunocytochemistry.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FITC

Fluorescein isothiocyante (FITC) is a derivative of fluorescein modified with an isothiocyanate reactive group (-N=C=S).  FITC is often used to label antibodies for immunofluorescent cytochemistry and histochemistry. FITC can be also conjugated to proteins and RNA and DNA probes because such conjugation does not impair biological activity of these molecules. In some experimental setups FITC fluorescence may need to be enhanced, which is done by employing anti-FITC antibodies conjugated either to FITC or other fluorophores with similar excitation and emission spectra.

Long Name
Fluorescein Isothiocyanate
Alternate Names
FITC; Fluorescein Isothiocyanate

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Citation for FITC Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Securidaca–saponins are natural inhibitors of AKT, MCL-1, and BCL2L1 in cervical cancer cells
    Authors: Titus Chukwuemeka Obasi, Cornelia Braicu, Bogdan Cezar Iacob, Ede Bodoki, Ancuta Jurj, Lajos Raduly et al.
    Cancer Management and Research

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