Human ASGR1/ASGPR1 Antibody

Catalog #: MAB4394 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB4394-100
MAB4394-SP

Detection of ASGR1/ASGPR1 in HepG2 Human Cell Line by Flow Cytometry.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human ASGR1/ASGPR1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human ASGR/1ASGPR1 in direct ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 950216

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human ASGR1/ASGPR1
Gln62-Leu291
Accession # P07306

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunocytochemistry

5-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of ASGR1/ASGPR1 in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human ASGR1/ASGPR1 Monoclonal Antibody (Catalog # MAB4394, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Immunocytochemistry

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ASGR1/ASGPR1 in BG01V Human Embryonic Stem Cells. ASGR1/ASGPR1 was detected in immersion fixed BG01V human embryonic stem cells differentiated to hepatocytes using Mouse Anti-Human ASGR1/ASGPR1 Monoclonal Antibody (Catalog # MAB4394) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Albumin was also detected using Goat Anti-Human/Mouse Serum Albumin Antibody (Catalog # AF3329). Cells were co-stained using the NorthernLights™ 493-conjugated Anti-Goat IgG Secondary Antibody (green; Catalog # NL003). Specific staining of ASGR1/ ASGPR1 was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ASGR1/ASGPR1

The human asialoglycoprotein receptor (ASGPR) is an endocytic recycling receptor that belongs to the long-form subfamily of the C-type/Ca⁺²-dependent lectin family (1, 2, 3). It is a complex of two noncovalently-linked subunits, a major 46 kDa glycoprotein (ASGR1) and a minor 50 kDa glycoprotein (ASGR2). The major human ASGPR subunit, ASGR1 (also H1), is synthesized as a 291 amino acid (aa) type II transmembrane (TM) glycoprotein. It contains a 40 aa cytoplasmic region, a 21 aa TM segment, and a 230 aa extracellular domain (ECD) (4 - 6). The cytoplasmic region contains one palmitoylation site at Cys36 that is essential for ligand endocytosis and dissociation (7). The ECD contains two important structural regions. The first is a stalk region of 62 aa (aa 61 - 123) that contributes to noncovalent oligomerization. The second is a 118 aa, carbohydrate-binding, Ca⁺²-dependent C-type lectin domain (aa 161 - 278) that is stabilized by three Ca⁺²ions (3, 8). Human ASGR1 ECD is 79% aa identical to mouse ASGR1 ECD. There are two minor (ASGR2) subunits that interact with ASGR1/H1 in a mutually exclusive manner to generate a functional ASGPR (9). They represent alternate splice forms of a type II TM protein. Termed H2b and H2c, H2b differs from H2c only by the presence of a 19 aa insert in its cytoplasmic region. This insert is significant because it allows serine phosphorylation of the cytoplasmic tail and provides for the majority of ASGPR ligand internalization (9). The stoichiometry of a functional ASGPR is unclear, but is suggested to be either a 2:2, 3:1 or 3:2 ratio of ASGR1/H1:ASGR2/H2 (9, 10, 11). ASGPR is found on hepatocytes and a subset of T cells (6, 12). ASGPR is reported to bind Gal (nonreducing), GalNAc, and sialic acid alpha 2,6Gal and GalNAc (3, 13, 14, 15). This is generally within the context of triantennary or tetraantennary configurations (2). The sialic acid terminations are of particular interest because molecules with these motifs most likely represent the endogenous ligands for ASGPR (14).

References

Stockert, R. J. (1995) Physiol. Rev. 75:591.
Weigel, P.H. and J.H.N. Yik (2002) Biochim. Biophys. Acta 1572:341.
Meier, M. et al. (2000) J. Mol. Biol. 300:857.
Spiess, M. et al. (1985) J. Biol. Chem. 260:1979.
Spiess, M. and H.F. Lodish (1986) Cell 44:177.
Bischoff, J. et al. (1988) J. Cell Biol. 106:1067.
Yik, J.H.N. et al. (2002) J. Biol. Chem. 277:40844.
Monroe, R.S. and B.E. Huber (1994) Gene 148:237.
Yik, J.H.N. et al. (2002) J. Biol. Chem. 277:23076.
Bider, M.D. et al. (1996) J. Biol. Chem. 271:31996.
Lodish, H. (1991) Trends Biochem. Sci. 16:374.
Park, J-H. et al. (2006) Biotechnol. Lett. 28:1061.
Westerlind, U. et al. (2004) Glyconj. J. 21:227.
Park, E.I. et al. (2005) Proc. Natl. Acad. Sci. USA 102:17125.
Park, E.I. et al. (2003) J. Biol. Chem. 278:4597.

Long Name

Asialoglycoprotein Receptor 1

Entrez Gene IDs

432 (Human); 11889 (Mouse); 24210 (Rat)

Alternate Names

ASGPR 1; ASGP-R 1; ASGPR; ASGPR1; ASGR1; asialoglycoprotein receptor 1; CLEC4H1; CLEC4H1ASGPR1; C-type lectin domain family 4 member H1; Hepatic lectin H1; HL-1; MHL1; RHL1