Human beta-Actin Antibody Summary
Accession # P60709
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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beta ‑Actin in HeLa Human Cell Line. beta -Actin was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human beta -Actin Monoclonal Antibody (Catalog # MAB8969) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: beta-Actin
Actin makes
up the most abundant protein in many eukaryotic cell types. It polymerizes to
form microfilaments that have an array of functions, including regulating
contractility, motility, cytokinesis, phagocytosis, adhesion, cell morphology,
and providing structural support. At least six types of Actin, falling into
three categories, exist in higher eukaryotes. The three types, separated based on
isoelectric point, include alpha ( alpha
-Skeletal, alpha -Cardiac, alpha -Smooth
Muscle), beta ( beta -Non-muscle) and gamma
( gamma -Smooth Muscle, gamma -non-muscle) isoforms. Non-muscle
beta - and gamma -actin are also known as cytoplasmic actin.
Product Datasheets
Citations for Human beta-Actin Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 5
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LncCDH5-3:3 Regulates Apoptosis, Proliferation, and Aggressiveness in Human Lung Cancer Cells
Authors: K Kwa?niak, J Czarnik-Kw, K Malysheva, K Pogoda, O Korchynsky, P Rybojad, B Karczmarek, J Tabarkiewi
Cells, 2022-01-23;11(3):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Expression and function of Toll‑like receptors in peripheral blood mononuclear cells in patients with ankylosing spondylitis
Authors: Jun Zhang, Rongming Xu, Lei Wu, Jihong Jiang
Molecular Medicine Reports
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The protective effects of total paeony glycoside on ischemia/reperfusion injury in H9C2 cells via inhibition of the PI3K/Akt signaling pathway
Authors: P Shen, J Chen, M Pan
Mol Med Rep, 2018-07-30;18(3):3332-3340.
Species: Rat
Sample Types: Cell Lysates
Applications: Western Blot -
Protective effect of KLF15 on vascular endothelial dysfunction induced by TNF??.
Authors: Bing Liu, Lili Xu, Xinming Yu, Wei Li, Xiaozhi Sun, Shun Xiao, Mingjin Guo, Haofu Wang
Molecular Medicine Reports, 2018-06-20;0(0):1791-3004.
Species: Human
Sample Types: Cell Lysate, Cell Lysates
Applications: Western Blot -
Effect of photodynamic therapy combined with Celecoxib on expression of cyclooxygenase-2 protein in HeLa cells
Authors: Y Mao, L Wang, C Xu, S Han
Oncol Lett, 2018-03-02;15(5):6599-6603.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
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