Human Carbohydrate Sulfotransferase 1/CHST1 Antibody

Catalog #: MAB5316
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB5316
MAB5316-SP

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All Carbohydrate Sulfotransferase 1/CHST1 Antibodies

Product Details

Citations (1)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Carbohydrate Sulfotransferase 1/CHST1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Carbohydrate Sulfotransferase 1/CHST1 is direct ELISAs. In direct ELISAs, approximately 25-100% cross-reactivity with recombinant mouse CHST1 and no cross-reactivity with recombinant human CHST2, 3, 4, 5, 6, 7, 10, and 14 is observed.

Source

Monoclonal Mouse IgG₁ Clone # 543128

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Carbohydrate Sulfotransferase 1/CHST1
Arg24-Ser411
Accession # O43916

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Immunoprecipitation

Neutralization

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Carbohydrate Sulfotransferase 1/CHST1

The CHST family is comprised of 14 enzymes in humans. All members of this family are Golgi-localized type II membrane proteins. Only the luminal and enzymatic domain is expressed in each of our recombinant CHST proteins. These enzymes transfer sulfate (i.e., sulfonate) onto the 6-O or 4-O positions of GalNAc, Gal and GlcNAc residues on glycoproteins, proteoglycans and glycolipids (1). This sulfation often creates specific epitopes that can be recognized by extracellular matrix proteins, cell surface receptors and viruses (2). CHST1, also known as keratan sulfate Gal-6 sulfotransferase, transfers sulfate to position 6 of galactose residues on keratan sulfate (3). It also has sulfotransferase activity on sialyl N-acetyllactosamine structures and participates in biosynthesis of selectin ligands that play a central role in lymphocyte homing at sites of inflammation (4). Human CHST1 shares 94% amino acid sequence identity with mouse CHST1.

Entrez Gene IDs

8534 (Human); 76969 (Mouse); 295934 (Rat)

Alternate Names

6-O-sulfotransferase 1; C6ST; carbohydrate (chondroitin 6/keratan) sulfotransferase 1; carbohydrate (keratan sulfate Gal-6) sulfotransferase 1; Carbohydrate Sulfotransferase 1; CHST1; EC 2.8.2; Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 1; GST-1; Keratan sulfate Gal-6 sulfotransferase; KS6ST; KSGal6ST; KSGal6STEC 2.8.2.21; KSST

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human Carbohydrate Sulfotransferase 1/CHST1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1