Human CD45 Antibody Summary
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
Detection of CD45 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD45 Monoclonal Antibody (Catalog # MAB1430, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.
CD45 in Human PBMCs. CD45 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with PHA blast using Mouse Anti-Human CD45 Monoclonal Antibody (Catalog # MAB1430) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Detection of CD45 in THP-1 Human Cell Line by Flow Cytometry. THP-1 human acute monocytic leukemia cell line was stained with Mouse Anti-Human CD45 Monoclonal Antibody (Catalog # MAB1430, filled histogram) or isotype control antibody (MAB002), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (F0102B). Staining was performed using our Staining Membrane-associated Proteins protocol.
CD45 Specificity is Shown by Flow Cytometry in Knockout Cell Line. CD45 knockout THP-1 human acute monocytic leukemia cell line was stained with Mouse Anti-Human CD45 Monoclonal Antibody (Catalog # MAB1430, filled histogram) or isotype control antibody (MAB002) followed by PE-conjugated anti-Mouse IgG Secondary Antibody (F0102B). No staining in the CD45 knockout THP-1 cell line was observed. Staining was performed using our Staining Membrane-associated Proteins protocol.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD45
CD45, previously called LCA (leukocyte common antigen), T200, or Ly5 in mice, is member C of the class 1 (receptor‑like) protein tyrosine phosphatase family (PTPRC) (1, 2). It is a variably glycosylated 180‑220 kDa transmembrane protein that is abundantly expressed on all nucleated cells of hematopoietic origin (1‑3). CD45 has several isoforms, expressed according to cell type, developmental stage and antigenic exposure (1‑5). The longest form, CD45RABC (called B220 in mouse), is expressed on B lymphocytes (5). The CD45RABC cDNA encodes 1304 amino acids (aa), including a 23 aa signal sequence, a 552 aa extracellular domain containing the splicing region, a cysteine‑rich region and two fibronectin type III domains, a 22 aa transmembrane sequence, and a 707 aa cytoplasmic domain that contains two phosphatase domains, D1 and D2. Only D1 has phosphatase activity. CD45R0 is the shortest form, lacking exons 4, 5 and 6 which encode aa 32‑191. It is expressed on memory cells, while intermediate sizes are expressed on other T cells (3, 4, 6). CD45 has been best studied in T cells, where it determines T cell receptor signaling thresholds (3, 6‑8). CD45 is moved into or out of the immunological synapse (IS) membrane microdomain depending on the relative influence of interaction with the extracellular galectin lattice or the intracellular actin cytoskeleton (9, 10). Galectin interaction can be fine‑tuned by varying usage of the heavily O‑glycosylated spliced regions and sialylation of N‑linked carbohydrates (4, 9). Within the IS, CD45 dephosphorylates and negatively regulates the Src family kinase, Lck (8‑10). In other leukocytes, CD45 influences differentiation and links immunoreceptor signaling with cytokine secretion and cell survival, partially overlapping in function with DEP‑1/CD148 (11‑14). CD45 deletion causes in severe immunodeficiency, while point mutations may be associated with autoimmune disorders (6, 7).
- Anderson, J.N. et al. (2004) FASEB J. 18:8.
- Streuli, M. et al. (1987) J. Exp. Med. 166:1548.
- Hermiston, M.L. et al. (2003) Annu. Rev. Immunol. 21:107.
- Earl, L.A. and L.G. Baum (2008) Immunol. Cell Biol. 86:608.
- Ralph, S.J. et al. (1987) EMBO J. 6:1251.
- Falahti, R. and D. Leitenberg (2008) J. Immunol. 181:6082.
- Tchilian, E.Z. and P.C.L. Beverley (2006) Trends Immunol. 27:146.
- McNiell, L. et al. (2007) Immunity 27:425.
- Chen, I-J. et al. (2007) J. Biol. Chem. 282:35361.
- Freiberg, B.A. et al. (2002) Nat. Immunol. 3:911.
- Zhu, J.W. et al. (2008) Immunity 28:183.
- Huntington, N.D. et al. (2006) Nat. Immunol. 7:190.
- Hesslein, D.G. et al. (2006) Proc. Natl. Acad. Sci. USA 103:7012.
- Cross, J.L. et al. (2008) J. Immunol. 180:8020.
Product Datasheets
Citations for Human CD45 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
19
Citations: Showing 1 - 10
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Regenerative Metaplastic Clones in COPD Lung Drive Inflammation and Fibrosis
Authors: Wei Rao, Shan Wang, Marcin Duleba, Suchan Niroula, Kristina Goller, Jingzhong Xie et al.
Cell
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Identification of ligand-receptor pairs that drive human astrocyte development
Authors: Voss, AJ;Lanjewar, SN;Sampson, MM;King, A;Hill, EJ;Sing, A;Sojka, C;Bhatia, TN;Spangle, JM;Sloan, SA;
Nature neuroscience
Species: Human
Sample Types: Whole Cells
Applications: Immunopanning -
Model of collective detachment in high-grade serous ovarian cancer demonstrates that tumor spheroids produce ECM to support metastatic processes
Authors: Hannah M. Micek, Lauren Rosenstock, Yicheng Ma, Caitlin Hielsberg, Lauren Montemorano, Metti K. Gari et al.
APL Bioengineering
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Vascular inflammation on a chip: A scalable platform for trans-endothelial electrical resistance and immune cell migration
Authors: Haley Ehlers, Arnaud Nicolas, Frederik Schavemaker, Jeroen P. M. Heijmans, Martin Bulst, Sebastiaan J. Trietsch et al.
Frontiers in Immunology
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A near infrared light-triggerable modular formulation for the delivery of small biomolecules
Authors: Vitor Francisco, Miguel Lino, Lino Ferreira
Journal of Nanobiotechnology
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Methionyl-tRNA Synthetase is a Useful Diagnostic Marker for Lymph Node Metastasis in Non-Small Cell Lung Cancer
Authors: Jung Mo Lee, Taehee Kim, Eun Young Kim, Arum Kim, Dong Ki Lee, Nam Hoon Kwon et al.
Yonsei Medical Journal
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Optimal design, anti-tumour efficacy and tolerability of anti-CXCR4 antibody drug conjugates
Authors: MJ Costa, J Kudaravall, JT Ma, WH Ho, K Delaria, C Holz, A Stauffer, AG Chunyk, Q Zong, E Blasi, B Buetow, TT Tran, K Lindquist, M Dorywalska, A Rajpal, DL Shelton, P Strop, SH Liu
Sci Rep, 2019-02-21;9(1):2443.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Fluoxetine induces direct inhibitory effects on mesenchymal stem cell?derived osteoprogenitor cells independent of serotonin concentration
Authors: SM Koura, M Salama, M El-Hussiny, MEA Khalil, A Lotfy, SA Hassan, SA Gad Elhak, MA Sobh
Mol Med Rep, 2019-02-01;0(0):.
Species: Rat
Sample Types: Whole Cells
Applications: Flow Cytometry -
FGL2 promotes tumor progression in the CNS by suppressing CD103+ dendritic cell differentiation
Authors: J Yan, Q Zhao, K Gabrusiewi, LY Kong, X Xia, J Wang, M Ott, J Xu, RE Davis, L Huo, G Rao, SC Sun, SS Watowich, AB Heimberger, S Li
Nat Commun, 2019-01-25;10(1):448.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Potential role of CBX7 in regulating pluripotency of adult human pluripotent-like olfactory stem cells in stroke model
Authors: JR Fan, HT Lee, W Lee, CH Lin, CY Hsu, CH Hsieh, WC Shyu
Cell Death Dis, 2018-05-01;9(5):502.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Blockade of Tumor-Expressed PD-1 promotes lung cancer growth
Authors: S Du, N McCall, K Park, Q Guan, P Fontina, A Ertel, T Zhan, AP Dicker, B Lu
Oncoimmunology, 2018-01-29;7(4):e1408747.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Regional Differences Between Perisynovial and Infrapatellar Adipose Tissue Depots and Their Response to Class II and III Obesity in Patients with OA
Authors: NS Harasymowi, ND Clement, A Azfer, R Burnett, DM Salter, AH Simpson
Arthritis & rheumatology (Hoboken, N.J.), 2017-06-10;0(0):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Proteomic profiling of peripheral blood neutrophils identifies two inflammatory phenotypes in stable COPD patients
Authors: ALT Loi, S Hoonhorst, C van Aalst, J Langereis, V Kamp, S Sluis-Eisi, N Ten Hacken, JW Lammers, L Koenderman
Respir. Res., 2017-05-22;18(1):100.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Preferential Lineage-Specific Differentiation of Osteoblast-Derived Induced Pluripotent Stem Cells into Osteoprogenitors
Authors: CL Roberts, SS Chen, AC Murchison, RA Ogle, MP Francis, RC Ogle, PC Sachs
Stem Cells Int, 2017-01-30;2017(0):1513281.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Feasibility and Efficiency of Human Bone Marrow Stromal Cell Culture with Allogeneic Platelet Lysate-Supplementation for Cell Therapy against Stroke
Stem Cells Int, 2016-10-20;2016(0):6104780.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Switch from canonical to noncanonical Wnt signaling mediates high glucose-induced adipogenesis.
Authors: Keats E, Dominguez J, Grant M, Khan Z
Stem Cells, 2014-06-01;32(6):1649-60.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Isolation and characterization of synovial mesenchymal stem cells.
Authors: Harvanova D, Tothova T, Sarissky M, Amrichova J, Rosocha J
Folia Biol. (Praha), 2011-01-01;57(3):119-24.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
The exosomal integrin alpha5beta1/AEP complex derived from epithelial ovarian cancer cells promotes peritoneal metastasis through regulating mesothelial cell proliferation and migration
Authors: Li X, Tang M, Zhu Q et al.
Cell Oncol (Dordr)
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Potentials and capabilities of the Extracellular Vesicle (EV) Array.
Authors: Jorgensen MM, Baek R, Varming K.
J Extracell Vesicles
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