Human Cytosolic Sulfotransferase 2A1/SULT2A1 Antibody

Catalog #: MAB5828 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB5828
MAB5828-SP

Detection of Human Cytosolic Sulfotransferase 2A1/SULT2A1 by Western Blot.

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All Cytosolic Sulfotransferase 2A1/SULT2A1 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Cytosolic Sulfotransferase 2A1/SULT2A1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Cytosolic Sulfotransferase 2A1/SULT2A1 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human SULT1A1, 1A3, 4A1, 1B1, 2B1, or 1E1 is observed.

Source

Monoclonal Mouse IgG_2A Clone # 656229

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human Cytosolic Sulfotransferase 2A1/SULT2A1
Ser2-Glu285
Accession # NP_003158

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Cytosolic Sulfotransferase 2A1/SULT2A1 by Western Blot. Western blot shows lysates of human liver tissue. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human Cytosolic Sulfotransferase 2A1/ SULT2A1 Mono-clonal Antibody (Catalog # MAB5828) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Cytosolic Sulfotransferase 2A1/SULT2A1 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry

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Cytosolic Sulfotransferase 2A1/SULT2A1 in HepG2 Human Cell Line. Cytosolic Sulfotransferase 2A1/SULT2A1 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human Cytosolic Sulfotransferase 2A1/ SULT2A1 Monoclonal Antibody (Catalog # MAB5828) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cytosolic Sulfotransferase 2A1/SULT2A1

Sulfotransferase 2A1 (SULT2A1), also called DHEA-ST (DHEA/bile acid sulfotransferase), is a 35 kDa member of the cytosolic sulfotransferase family, which are Phase II drug-metabolizing enzymes that catalyze the sulfation of many endogenous and xenobiotic substrates. SULT2A1 is primarily expressed in liver and adrenal tissues. It sulfates steroids and bile acids, playing roles in maintaining cholesterol and bile acid homeostasis by increasing cholesterol catabolism and preventing toxicity from bile acid accumulation. Human SULT2A1 shares 63-65% amino acid identity with mouse and rat SULT2A1.

Entrez Gene IDs

6822 (Human); 20859 (Mouse); 24912 (Rat)

Alternate Names

alcohol/hydroxysteroid sulfotransferase; Cytosolic Sulfotransferase 2A1; Dehydroepiandrosterone sulfotransferase; DHEAS; DHEA-ST; DHEA-STbile salt sulfotransferase; EC 2.8.2.14; HST; hSTa; Hydroxysteroid Sulfotransferase; ST2; ST2A1; ST2A3; STDbile-salt sulfotranasferase 2A1; Sulfotransferase 2A1; sulfotransferase family, cytosolic, 2A, dehydroepiandrosterone(DHEA)-preferring, member 1; SULT2A1