Endothelial NOS (eNOS), also known as nitric oxide synthase 3 (NOS3) or constitutive NOS (cNOS), is an enzyme encoded by the NOS3 gene. Endothelial NOS generates nitric oxide in blood vessels and is involved with regulating vascular tonehttp://en.wikipedia.org/wiki/Vascular_resistance" title="Vascular resistance"> by inhibiting smooth muscle contraction and platelethttp://en.wikipedia.org/wiki/Platelet" title="Platelet"> aggregation. A constitutive calciumhttp://en.wikipedia.org/wiki/Calcium_in_biology" title="Calcium in biology"> dependent NOS provides a basal release of NO. eNOS is associated with plasma membranes surrounding cells and the membranes of the Golgi apparatushttp://en.wikipedia.org/wiki/Golgi_apparatus" title="Golgi apparatus"> within cells.
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Scientific Data Images for Human eNOS Antibody
Detection of Human eNOS by Western Blot.
Western blot shows lysates of HUVEC human umbilical vein endothlial cells. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human eNOS Affinity-purified Polyclonal Antibody (Catalog # AF950) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for eNOS at approximately 130-140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Immunoprecipitation of Human eNOS.
eNOS was immunoprecipitated from lysates (1 x 106cells) of HUVEC human umbilical vein endothelial cells following incubation with 0.3 µg Goat Anti-Human eNOS Antigen Affinity-purified Polyclonal Antibody (Catalog # AF950). Immunoprecipitated eNOS was detected by Western blot using 1 µg/mL Human eNOS Antigen Affinity-purified Polyclonal Antibody (Catalog # AF950). View our recommended buffer recipes for immunoprecipitation.
Detection of Human eNOS by Simple WesternTM.
Simple Western lane view shows lysates of HUVEC human umbilical vein endothelial cells, loaded at 0.2 mg/mL. A specific band was detected for eNOS at approximately 139 kDa (as indicated) using 10 µg/mL of Goat Anti-Human eNOS Antigen Affinity-purified Polyclonal Antibody (Catalog # AF950) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Detection of Human eNOS by Western Blot
Phenolic metabolites upregulate eNOS pathway in response to TNF-alpha. The expression and phosphorylation of eNOS was measured in HUVECs pre-stimulated with C3G and phenolic metabolites for 24 h +/− TNF-alpha for an additional 24 h. (A) Representative graphs for eNOS mRNA. (B) Representative Western blot bands for eNOS. (C) Western blot representative graphs. All data expressed as mean = 3, ±SEM. Significance value was set at # p < 0.05, ## p < 0.01 vs. control and * p < 0.05 versus TNF-alpha. Image collected and cropped by CiteAb from the following open publication (https://www.mdpi.com/2218-1989/14/11/613), licensed under a CC-BY license. Not internally tested by R&D Systems.Applications for Human eNOS Antibody
Immunohistochemistry
Sample: Immersion fixed paraffin-embedded sections of human placenta subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)
Immunoprecipitation
Sample: HUVEC human umbilical vein endothelial cells, see our available Western blot detection antibodies
Simple Western
Sample: HUVEC human umbilical vein endothelial cells
Western Blot
Sample: eNOS immunoprecipitated HUVEC human umbilical vein endothelial cells
Formulation, Preparation, and Storage
Purification
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: eNOS
Long Name
Alternate Names
Gene Symbol
Additional eNOS Products
Product Documents for Human eNOS Antibody
Product Specific Notices for Human eNOS Antibody
For research use only
Related Research Areas
Citations for Human eNOS Antibody
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
