Human Erythropoietin/EPO Antibody Summary
Accession # P01588
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Cell Proliferation Induced by Erythropoietin/EPO and Neutralization by Human Erythropoietin/EPO Antibody. Recombinant Human Erythropoietin/EPO (Tissue Culture Grade) (Catalog # 287-TC) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Erythropoietin/EPO (Tissue Culture Grade) (0.3 units/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human Erythropoietin/EPO Monoclonal Antibody (Catalog # MAB287). The ND50 is typically 0.5-1.5 µg/mL.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Erythropoietin/EPO
Erythropoietin (EPO) a glycoprotein produced primarily by the kidney, is the principal factor that regulates erythropoiesis by stimulating the proliferation and differentiation of erythroid progenitor cells.
Product Datasheets
Citations for Human Erythropoietin/EPO Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Continuous Translation of Circularized mRNA Improves Recombinant Protein Titer
Authors: A Costello, NT Lao, N Barron, M Clynes
Metab. Eng., 2019-01-04;0(0):.
Species: Transgenic Hamster
Sample Types: Cell Culture Supernates
Applications: ELISA Development (Capture) -
Effect of erythropoietin on primed leucocyte expression profile
Authors: Mirko Pesce, Paolo Felaco, Sara Franceschelli, Lorenza Speranza, Alfredo Grilli, Maria Anna Anna De Lutiis et al.
Open Biology
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Novel human renal proximal tubular cell line for the production of complex proteins.
Authors: Fliedl L, Manhart G, Kast F, Katinger H, Kunert R, Grillari J, Wieser M, Grillari-Voglauer R
J Biotechnol, 2014-02-16;176(0):29-39.
Species: Human
Sample Types: Recombinant Protein
Applications: ELISA Development (Capture) -
The role and regulation of erythropoietin (EPO) and its receptor in skeletal muscle: how much do we really know?
Authors: Séverine Lamon, Aaron P. Russell
Frontiers in Physiology
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Macrophages as novel target cells for erythropoietin
Authors: Lilach Lifshitz, Galit Tabak, Max Gassmann, Moshe Mittelman, Drorit Neumann
Haematologica
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Erythropoietin exerts anti-epileptic effects with the suppression of aberrant new cell formation in the dentate gyrus and upregulation of neuropeptide Y in seizure model of rats.
Authors: Kondo A, Shingo T, Yasuhara T, Kuramoto S, Kameda M, Kikuchi Y, Matsui T, Miyoshi Y, Agari T, Borlongan CV, Date I
Brain Res., 2009-08-18;1296(0):127-36.
Species: Rat
Sample Types: In Vivo
Applications: Neutralization -
Erythropoietin inhibits respiratory epithelial cell apoptosis in a model of acute lung injury.
Authors: MacRedmond R, Singhera GK, Dorscheid DR
Eur. Respir. J., 2009-01-22;33(6):1403-14.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Functional and immunochemical characterisation of different antibodies against the erythropoietin receptor.
Authors: Kirkeby A, van Beek J, Nielsen J, Leist M, Helboe L
J Neurosci Methods, 2007-04-12;164(1):50-8.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Western Blot -
Multiplexed digital mRNA expression analysis profiles system-wide changes in mRNA abundance and responsiveness of UPR-specific gene expression changes during batch culture of recombinant Chinese Hamster Ovary cells
Authors: R Maldonado-, AJ Dickson
Biotechnol J, 2018-02-02;0(0):.
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Erythropoietin signaling increases neurogenesis and oligodendrogenesis of endogenous neural stem cells following spinal cord injury both in�vivo and in�vitro
Authors: H Zhang, X Fang, D Huang, Q Luo, M Zheng, K Wang, L Cao, Z Yin
Mol Med Rep, 2017-10-25;0(0):.
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