Human FGF-13 1B Antibody

Catalog #: MAB1909 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB1909
MAB1909-SP

Detection of FGF‑13 in RPMI 8226 Human Cell Line by Flow Cytometry.

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All FGF-13 Antibodies

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Human FGF-13 1B Antibody Summary

Species Reactivity

Human

Specificity

Detects human FGF-13 1B in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) FGF-3, -4, -5, -6, -7, -9, -10, -11, -12, -16, -17, -19, -20, -21, rhFGF basic, recombinant mouse FGF-8b, or -8c is observed.

Source

Monoclonal Mouse IgG_2A Clone # 298923

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human FGF-13 isoform 1B
Ala2-Thr192
Accession # NP_378668

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human FGF‑13 1B

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of FGF‑13 in RPMI 8226 Human Cell Line by Flow Cytometry. RPMI 8226 human multiple myeloma cell line was stained with Mouse Anti-Human FGF-13 1B Monoclonal Antibody (Catalog # MAB1909, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FGF-13

FGF-13 (Fibroblast Growth Factor-13), also known as FGF homologous factor 2 (FHF-2), is a member of the FGF family. It is expressed in brain and skeletal muscle and has been implicated in nervous system development and function. The gene for FGF-13 has been localized in human chromosome X, in a region associated with Borjeson-Forssman-Lehmann syndrome, a syndromal X-linked mental retardation. Two alternately spliced isoforms that differ in their amino-terminal residues have been described.

Long Name

Fibroblast Growth Factor 13

Entrez Gene IDs

2258 (Human)

Alternate Names

FGF13; FGF-13; FGF2Fibroblast growth factor homologous factor 2; FHF2FHF-2; fibroblast growth factor 13