Human FGF-19 Antibody

Catalog #: MAB9691 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB9691-SP
MAB9691-500
MAB9691-100

All FGF-19 Antibodies

Product Details

Supplemental Products

Reviews

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human FGF-19 Antibody Summary

Species Reactivity

Human

Specificity

Detects human FGF-19 in direct ELISAs and Western blots. Shows approximately 10% cross-reactivity with recombinant human (rh) FGF‑3 and rhFGF-5 and no cross-reactivity with rhFGF-4, rhFGF-6, rhFGF-7, rhFGF-9, rhFGF-10, rhFGF-13, rhFGF-16, rhFGF‑17, rhFGF-18, rhFGF acidic, and rhFGF basic.

Source

Monoclonal Mouse IgG₁ Clone # 117611

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human FGF-19
Phe27-Lys216
Accession # O95750

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human FGF‑19 (Catalog # 969-FG)
under non-reducing conditions only

Blockade of Receptor-ligand Interaction

In a functional ELISA, 0.7-4 μg/mL of this antibody will block 50% of the binding of 400 ng/mL of Recombinant Human FGF-19 (Catalog # 969-FG) to immobilized Recombinant Human FGF R4 Fc Chimera (Catalog # 685-FR) coated at 5 µg/mL (100 µL/well). At 10 μg/mL, this antibody will block >90% of the binding.

Human FGF-19 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

2-8 µg/mL

Use in combination with:

Detection Reagent: Human FGF‑19 Biotinylated Antibody (Catalog # BAF969)

Standard: Recombinant Human FGF-19 Protein (Catalog # 969-FG)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FGF-19

Fibroblast growth factor 19 (FGF-19) belongs to the large FGF family which has at least 23 members (1, 2). All FGF family members are heparin-binding growth factors with a core 120 amino acid (aa) FGF domain that allows for a common tertiary structure. FGFs are expressed during embryonic development and in restricted adult tissues. They act on cells of mesodermal and neuroectodermal origin to regulate diverse physiologic functions including angiogenesis, cell growth, pattern formation, embryonic development, metabolic regulation, cell migration, neurotrophic effects and tissue repair (3, 4). Signaling receptors for FGFs are type I transmembrane receptor tyrosine kinases belonging to the Ig superfamily. Four distinct but related classes of FGF receptors, FGF R1, 2, 3, and 4, exist. Through alternative splicing, multiple isoforms for FGF R1, 2 and 3, with distinct ligand recognition profiles, are also generated (4). Human FGF-19 cDNA predicts a 251 aa precursor protein with a 22 aa signal peptide and a 229 aa secreted mature protein with no potential N-linked glycosylation sites (1, 2). Among FGF family members, human FGF-19 is most closely related to chicken FGF-19 and murine FGF-15, sharing approximately 61% and 51% aa sequence identity, respectively (1, 2, 5). Neither the human orthologue of mouse FGF-15, nor the mouse counterpart of human FGF-19 has been identified. With the exception of adult gall bladder epithelium, FGF-19 expression is restricted to fetal tissues (1, 2). Unlike most FGFs which bind to and activate more than one FGF receptor, FGF-19 is a specific ligand for FGF R4 (2). Similarly, another FGF family member, FGF-7 (KGF), only activates KGF R, the IIIb isoform of FGF R2 (4). During chick embryogenesis, FGF-19 has been shown to act synergistically with Wnt-8c to initiate inner ear development (5).

References