Human Glycine N-methyltransferase/GNMT Antibody

Catalog #: MAB6526 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6526
MAB6526-SP

Detection of Mouse Glycine N-methyltransferase/GNMT by Western Blot.

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All Glycine N-Methyltransferase/GNMT Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Glycine N-methyltransferase/GNMT Antibody Summary

Species Reactivity

Human

Specificity

Detects human Glycine N-methyltransferase/GNMT in direct ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 691305

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human Glycine N-methyltransferase/GNMT
Met1-Asp295
Accession # Q14749

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunoprecipitation

25 µg/mL

Cell lysates spiked with recombinant human Glycine N-methyltransferase/GNMT, see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Mouse Glycine N-methyltransferase/GNMT by Western Blot. Western blot shows lysates of mouse liver tissue. PVDF Membrane was probed with 1 µg/mL of Human Glycine N-methyltransferase/GNMT Monoclonal Antibody (Catalog # MAB6526) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Glycine N-methyltransferase/GNMT at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Glycine N-Methyltransferase/GNMT

GNMT (glycine N-methyltransferase) is a cytoplasmic homotetramer of 33 kDa units that converts S-adenosylmethionine to N-methylglycine (sarcosine), also producing S-adenosylhomocysteine. It is abundant in liver, but has also been found in the pancreas and prostate, and is downregulated in hepatocellular carcinoma. GNMT adjusts liver methyl group synthesis for dietary methionine, influencing DNA methylation. The 5-methyltetrahydrofolate form of folic acid binds and inhibits GNMT activity. The 295 amino acid (aa) human GNMT shares 92% aa identity with mouse and rat GNMT.

Entrez Gene IDs

27232 (Human); 14711 (Mouse); 25134 (Rat)

Alternate Names

EC 2.1.1.20; Glycine NMethyltransferase; Glycine N-Methyltransferase; GNMT