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Human IgG3 Antibody

Catalog #: MAB10122 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB10122-100
MAB10122-SP
Detection of Human IgG3by Western Blot.
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Human IgG3 Antibody Summary

Species Reactivity
Human
Specificity
Detects human IgG3 in direct ELISAs and Western blots. In Western blots, no cross-recativity with human IgG1, human IgG2, and human IgG4 is observed.
Source
Monoclonal Mouse IgG2A Clone # 985438
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Purified human IgG3
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human IgG<sub>3</sub>by Western Blot. View Larger

Detection of Human IgG3by Western Blot. Western blot shows lysates of purified human IgG1(negative control), purified human IgG2(negative control), purified human IgG3, and human purified human IgG4(negative control). PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human IgG3Monoclonal Antibody (Catalog # MAB10122) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for IgG3at approximately 240 kDa (as indicated). Human IgG (Catalog # MAB1101) is shown as a loading control.This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IgG3

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G3
Alternate Names
DKFZp686H11213; FLJ39988; FLJ40036; FLJ40253; FLJ40587; FLJ40789; FLJ40834; IgG3; immunoglobulin heavy constant gamma 3 (G3m marker); MGC45809

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