Human IL-21R Antibody

Name: Human IL-21R Antibody
Brand: R&D Systems
SKU: MAB991
Rating: 5 (1 reviews)

Catalog #: MAB991
5 Citations 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB991
MAB991-SP

All IL-21R Antibodies

Product Details

Citations (5)

FAQs

Supplemental Products

Reviews (1)

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IL-21R Antibody Summary

Species Reactivity

Human

Specificity

Detects human IL-21 R in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross‑react with recombinant mouse IL‑21 R.

Source

Monoclonal Mouse IgG₁ Clone # 152504

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL-21 R
Cys20-Pro236
Accession # Q9HBE5

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human IL‑21 R Subunit Fc Chimera (Catalog # 991-R2)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-21R

Interleukin-21 (IL-21) and its receptor play an important role in the regulation of the immune system. IL-21 R, also called NILR (novel interleukin receptor), is a type I cytokine receptor with 4 conserved cysteine residues and an extracellular WSXWS motif. It is most closely related to IL-2 R beta, IL-4 R alpha, and IL-9 R. The gene for human IL-21 R has been mapped to chromosome 16p12. Human IL-21 R is a 538 amino acid (aa) residue type I transmembrane protein with a 19 aa signal peptide, a 217 aa extracellular domain, a 19 aa transmembrane domain and a 283 aa cytoplasmic domain. IL-21 R is expressed on lymphoid tissues, peripheral B cells and cell lines of T, B, and NK lineage. The common gamma chain ( gamma _c) is required for IL-21 R signaling. The IL-21/IL-21 R interaction appears to play an important role in B and T cell proliferation after antigen stimulation and NK cell maturation.

References

Parrish-Novak, et al. (2000) Nature 408:57.
Ozaki, K. et al. (2000) Proc. Natl. Acad. Sci. USA 97:11439.
Dumoutier, L. et al. (2000) Proc. Natl. Acad. Sci. USA 97:10144.
Asao, H. et al. (2001) J. Immunol. 167:1.

Long Name

Interleukin 21 Receptor

Entrez Gene IDs

50615 (Human); 60504 (Mouse); 102135042 (Cynomolgus Monkey); 705759 (Rhesus Macaque)

Alternate Names

CD360 antigen; CD360; IL-21 R; IL-21 receptor; IL21R; IL-21R; interleukin 21 receptor; interleukin-21 receptor; MGC10967; NILR; Novel interleukin receptor

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human IL-21R Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Activation of the FcgammaReceptorIIIa on human natural killer cells leads to increased expression of functional interleukin-21 receptor
Authors: EL McMichael, NB Courtney, MC Duggan, R Wesolowski, D Quiroga, SV Kondadasul, LS Atwal, N Bhave, E Luedke, AC Jaime-Rami, AR Campbell, X Mo, JC Byrd, WE Carson Iii
Oncoimmunology, 2017-05-02;6(5):e1312045.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Dual role of IL-21 in megakaryopoiesis and platelet homeostasis
Authors: S Benbarche, C Strassel, C Ang‚nieux, L Mallo, M Freund, C Gachet, F Lanza, H de la Sall
Haematologica, 2017-01-05;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Loss-of-function mutations in the IL-21 receptor gene cause a primary immunodeficiency syndrome.
Authors: Kotlarz D, Zietara N, Uzel G, Weidemann T, Braun C, Diestelhorst J, Krawitz P, Robinson P, Hecht J, Puchalka J, Gertz E, Schaffer A, Lawrence M, Kardava L, Pfeifer D, Baumann U, Pfister E, Hanson E, Schambach A, Jacobs R, Kreipe H, Moir S, Milner J, Schwille P, Mundlos S, Klein C
J Exp Med, 2013-02-25;210(3):433-43.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Control of matrix metalloproteinase production in human intestinal fibroblasts by interleukin 21.
Authors: Monteleone G, Caruso R, Fina D, Peluso I, Gioia V, Stolfi C, Fantini MC, Caprioli F, Tersigni R, Alessandroni L, MacDonald TT, Pallone F
Gut, 2006-05-08;55(12):1774-80.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Interleukin-21 receptor (IL-21R) is up-regulated by CD40 triggering and mediates proapoptotic signals in chronic lymphocytic leukemia B cells.
Authors: de Totero D, Meazza R, Zupo S, Cutrona G, Matis S, Colombo M, Balleari E, Pierri I, Fabbi M, Capaia M, Azzarone B, Gobbi M, Ferrarini M, Ferrini S
Blood, 2006-01-03;107(9):3708-15.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot