Home / Aminopeptidase PILS/ARTS1 / Human/Mouse Aminopeptidase PILS/ARTS1 Antibody

Human/Mouse Aminopeptidase PILS/ARTS1 Antibody

Catalog #: MAB2334
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB2334
MAB2334-SP
Detection of Human Aminopeptidase PILS/ARTS1 by Western Blot.
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Citations (3)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse Aminopeptidase PILS/ARTS1 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human Aminopeptidase PILS/ARTS1 in direct ELISAs and Western blots. In direct ELISAs, 100% cross-reactivity with recombinant mouse Aminopeptidase PILS/ARTS1 is observed.
Source
Monoclonal Mouse IgG1 Clone # 6H9
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
S. frugiperda insect ovarian cell line Sf 9-derived recombinant human ARTS1
Accession # Q9NZ08
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Simple Western
20 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Aminopeptidase PILS/ARTS1 antibody by Western Blot. View Larger

Detection of Human Aminopeptidase PILS/ARTS1 by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse Aminopeptidase PILS/ARTS1 Monoclonal Antibody (Catalog # MAB2334) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Aminopeptidase PILS/ARTS1 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western Detection of Human Aminopeptidase PILS/ARTS1 antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human Aminopeptidase PILS/ARTS1 by Simple WesternTM. Simple Western lane view shows lysates of A431 human epithelial carcinoma cell line and Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for Aminopeptidase PILS/ARTS1 at approximately 114 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human/Mouse Aminopeptidase PILS/ARTS1 Monoclonal Antibody (Catalog # MAB2334). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Aminopeptidase PILS/ARTS1

Aminopeptidase PILS/ARTS1 is also known as adipocyte-derived leucine aminopeptidase, type 1 tumor necrosis factor receptor shedding aminopeptidase regulator and ERAAP (the aminopeptidase associated with antigen processing in the endoplasmic reticulum). ARTS1 is widely expressed and releases an N-terminal amino acid (Leu). The amino acid sequence of mouse ARTS1 is 94%, 84%, 80 and 70% identical to that of rat, human, canine, and chicken.

Long Name
Aminopeptidase Puromycin-insensitive Leucyl-specific
Entrez Gene IDs
51752 (Human); 80898 (Mouse); 102146570 (Cynomolgus Monkey)
Alternate Names
Adipocyte-derived leucine aminopeptidase; A-LAP; A-LAPALAP; Aminopeptidase PILS; ARTS1; ARTS1aminopeptidase regulator of TNFR1 shedding; ARTS-1PILSAP; EC 3.4.11; EC 3.4.11.-; EC 3.4.11.1; endoplasmic reticulum aminopeptidase 1; endoplasmic reticulum aminopeptidase associated with antigen processing; ERAAP1; ERAP1; KIAA0525APPILS; PILS-AP; PILS-APERAAP; Puromycin-insensitive leucyl-specific aminopeptidase; Type 1 tumor necrosis factor receptor shedding aminopeptidase regulator

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Citations for Human/Mouse Aminopeptidase PILS/ARTS1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Editing the immunopeptidome of melanoma cells using a potent inhibitor of endoplasmic reticulum aminopeptidase 1 (ERAP1)
    Authors: Despoina Koumantou, Eilon Barnea, Adrian Martin-Esteban, Zachary Maben, Athanasios Papakyriakou, Anastasia Mpakali et al.
    Cancer Immunology, Immunotherapy
  2. ER-aminopeptidase 1 determines the processing and presentation of an immunotherapy-relevant melanoma epitope
    Authors: K Textoris-T, C Cammann, P Henklein, E Topfstedt, F Ebstein, S Henze, J Liepe, F Zhao, D Schadendor, B Dahlmann, W Uckert, A Paschen, M Mishto, U Seifert
    Eur. J. Immunol., 2019-11-27;0(0):.
    Species: Human
    Sample Types: Cell Lysa
    Applications: Western Blot
  3. The proteasome immunosubunits, PA28 and ER-aminopeptidase 1 protect melanoma cells from efficient MART-126-35 -specific T-cell recognition.
    Authors: Keller M, Ebstein F, Burger E, Textoris-Taube K, Gorny X, Urban S, Zhao F, Dannenberg T, Sucker A, Keller C, Saveanu L, Kruger E, Rothkotter H, Dahlmann B, Henklein P, Voigt A, Kuckelkorn U, Paschen A, Kloetzel P, Seifert U
    Eur J Immunol, 2015-10-19;45(12):3257-68.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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