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Human/Mouse BACE-1 Ectodomain Antibody

Catalog #: MAB931
15 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB931-SP
MAB931-500
MAB931-100
Detection of Human BACE‑1 by Western Blot.
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Product Details
Citations (15)
FAQs
Supplemental Products
Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse BACE-1 Ectodomain Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse BACE-1 in direct ELISAs and human BACE-1 in Western blots. In Western blots, no cross-reactivity with recombinant human (rh) BACE-2, recombinant mouse (rm) BACE-2, rhADAM8, rmADAM9, rmADAM10, rhADAM15, or rhTACE is observed.
Source
Monoclonal Mouse IgG1 Clone # 137612
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human BACE-1
Thr22-Tyr460
Accession # P56817
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Immunohistochemistry
8-25 µg/mL
Immersion fixed paraffin-embedded sections of human Alzheimer's disease brain
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
Jurkat human acute T cell leukemia cell line fixed with paraformaldehyde and permeabilized with saponin

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human BACE‑1 antibody by Western Blot. View Larger

Detection of Human BACE‑1 by Western Blot. Western blot shows lysates of human brain (Alzheimer's disease hippocampus) tissue. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse BACE-1 Ectodomain Monoclonal Antibody (Catalog # MAB931) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for BACE-1 at approximately 60 and 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BACE-1

BACE-1 (beta‑site APP cleaving enzyme-1) is an aspartic protease and an integral membrane protein (1, 2). It is the major beta secretase, and together with the gamma  secretase, is responsible for generating the amyloid beta peptide (A beta ) from the amyloid precursor protein (APP) (3, 4). Because A beta is a major component of amyloid plaques, BACE-1 has been implicated in the onset and/or progression of Alzheimer's disease. High levels of BACE-1 activity are sufficient to elicit neurodegeneration and neurological decline in vivo, indicating that inhibiting BACE-1 may block not only A beta -dependent but also A beta -independent pathogenic mechanisms (5). In addition to APP, BACE-1 also cleaves APP-like proteins 1 and 2, the cell adhesion protein P-selectin glycoprotein ligand-1 and beta -galactoside alpha 2,6-sialyltransferase, implying that BACE-1 may have additional functions involving the ectodomain shedding of membrane proteins (6‑8).

References
  1. Vassar, R. et al. (1999) Science 286:735.
  2. Yan, R. et al. (1999) Nature 402:533.
  3. Cai, H. et al. (2001) Nature Neurosci. 4:233.
  4. Roberds, S.L. et al. (2001) Human Mol. Genet. 97:1317.
  5. Rockenstein, E. et al. (2005) J. Biol. Chem. 280:32957.
  6. Li, Q and T.C. Sudhof (2004) J. Biol. Chem. 279:10542.
  7. Lichtenthaler, S.F. et al. (2003) J. Biol. Chem. 278:48713.
  8. Kitazynem, S. et al. (2005) J. Biol. Chem. 280:8589.
Long Name
beta-site Ayloid Precursor Protein Cleaving Enzyme 1
Entrez Gene IDs
23621 (Human); 23821 (Mouse)
Alternate Names
APP beta-secretase; ASP2; Aspartyl protease 2; BACE1; BACE-1; BACEAsp 2; beta-secretase 1 precursor variant 1; beta-secretase 1; beta-site amyloid beta A4 precursor protein-cleaving enzyme; Beta-site amyloid precursor protein cleaving enzyme 1; Beta-site APP cleaving enzyme 1; beta-site APP-cleaving enzyme 1; beta-site APP-cleaving enzyme; EC 3.4.23; EC 3.4.23.46; FLJ90568; HSPC104; KIAA1149; memapsin-2; Membrane-associated aspartic protease 2; transmembrane aspartic proteinase Asp2

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Citations for Human/Mouse BACE-1 Ectodomain Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

15 Citations: Showing 1 - 10
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  1. Impact of increased APP gene dose in Down syndrome and the Dp16 mouse model
    Authors: Mariko Sawa, Cassia Overk, Ann Becker, Dominique Derse, Ricardo Albay, Kim Weldy et al.
    Alzheimer's & Dementia
  2. Green Tea Seed Isolated Theasaponin E1 Ameliorates AD Promoting Neurotoxic Pathogenesis by Attenuating Abeta Peptide Levels in SweAPP N2a Cells
    Authors: MI Khan, JH Shin, MY Kim, TS Shin, JD Kim
    Molecules, 2020-05-16;25(10):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Tracking extracellular vesicle phenotypic changes enables treatment monitoring in melanoma
    Authors: J Wang, A Wuethrich, AA Sina, RE Lane, LL Lin, Y Wang, J Cebon, A Behren, M Trau
    Sci Adv, 2020-02-26;6(9):eaax3223.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Characterization of lysosomal proteins Progranulin and Prosaposin and their interactions in Alzheimer's disease and aged brains: increased levels correlate with neuropathology
    Authors: A Mendsaikha, I Tooyama, JP Bellier, GE Serrano, LI Sue, LF Lue, TG Beach, DG Walker
    Acta Neuropathol Commun, 2019-12-21;7(1):215.
    Species: Human
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC-Fr, Western Blot
  5. Increased Plasma Beta-Secretase 1 May Predict Conversion to Alzheimer’s Disease Dementia in Individuals With Mild Cognitive Impairment
    Authors: Yong Shen, Haibo Wang, Qiying Sun, Hailan Yao, Andrew P. Keegan, Mike Mullan et al.
    Biological Psychiatry
  6. Deficiency of Neuronal p38alpha MAPK Attenuates Amyloid Pathology in Alzheimer Disease Mouse and Cell Models through Facilitating Lysosomal Degradation of BACE1.
    Authors: Schnoder L, Hao W, Qin Y, Liu S, Tomic I, Liu X, Fassbender K, Liu Y
    J Biol Chem, 2015-12-09;291(5):2067-79.
    Species: Human
    Sample Types: Tissue Homogenates, Whole Cells
    Applications: IHC, Western Blot
  7. Overexpression of the IGF-II/M6P receptor in mouse fibroblast cell lines differentially alters expression profiles of genes involved in Alzheimer's disease-related pathology.
    Authors: Wang Y, Thinakaran G, Kar S
    PLoS ONE, 2014-05-20;9(5):e98057.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. High activities of BACE1 in brains with mild cognitive impairment.
    Authors: Cheng X, He P, Lee T, Yao H, Li R, Shen Y
    Am J Pathol, 2014-01-01;184(1):141-7.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: ELISA Development, Western Blot
  9. Chronic Administration of Anti-Stroke Herbal Medicine TongLuoJiuNao Reduces Amyloidogenic Processing of Amyloid Precursor Protein in a Mouse Model of Alzheimer’s Disease
    Authors: Ping He, Pengtao Li, Qian Hua, Yuan Liu, Matthias Staufenbiel, Rena Li et al.
    PLoS ONE
  10. Long-Term Treatment of Thalidomide Ameliorates Amyloid-Like Pathology through Inhibition of beta -Secretase in a Mouse Model of Alzheimer’s Disease
    Authors: Ping He, Xin Cheng, Matthias Staufenbiel, Rena Li, Yong Shen
    PLoS ONE
  11. A beta aggregation profiles and shifts in APP processing favor amyloidogenesis in canines
    Authors: Viorela Pop, Elizabeth Head, Nicole C. Berchtold, Charles G. Glabe, Christa M. Studzinski, Adam M. Weidner et al.
    Neurobiology of Aging
  12. Development of a specific ELISA to measure BACE1 levels in human tissues
    Authors: Amanda Gonzales, Boris Decourt, Aaron Walker, Rachel Condjella, Hikmet Nural, Marwan N. Sabbagh
    Journal of Neuroscience Methods
  13. p75NTR regulates Abeta deposition by increasing Abeta production but inhibiting Abeta aggregation with its extracellular domain
    Authors: Yan-Jiang Wang, Xin Wang, Jian-Jun Lu, Qiao-Xin Li, Chang-Yue Gao, Xiao-Hong Liu et al.
    The Journal of Neuroscience
  14. Changes in Cognition and Amyloid-beta Processing with Long Term Cholesterol Reduction using Atorvastatin in Aged Dogs
    Authors: M. Paul Murphy, Jacqueline Morales, Tina L. Beckett, Giuseppe Astarita, Daniele Piomelli, Adam Weidner et al.
    Journal of Alzheimer's Disease
  15. BACE1 and BACE2 enzymatic activities in Alzheimer's disease.
    Authors: Ahmed RR, Holler CJ, Webb RL
    J. Neurochem., 2009-12-04;112(4):1045-53.
    Species: Human, Rat
    Sample Types: Tissue Homogenates
    Applications: ELISA Development, Immunoprecipitation, Western Blot

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Human/Mouse BACE-1 Ectodomain Antibody
By Anonymous on 03/07/2018
Application: Func Sample Tested: Human recombinant antibody Species: Human