Human/Mouse G9a/EHMT2 Antibody Summary
aa 15-114
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Mouse G9a/EHMT2 by Western Blot G9a is not required for the transcriptional repression of selected early embryonic genes during retinoic acid (RA)-induced differentiation. The indicated mESC lines were untreated (0) or treated with 1 μM RA for 2, 4 or 8 days. (A) Immunoblots were used to determine G9a, Oct3/4 and GAPDH (loading control) protein levels in whole cell extracts. (B) Graph shows the messenger (mRNA) levels for the indicated genes using RT-qPCR analysis with Ubiquitin C (UBC) as the normalization control. All mRNA levels are normalized to the TT2 0 RA sample. Results shown are mean ± SD for three PCR reactions performed on the same cDNA sample and are representative of three independent experiments. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25478012), licensed under a CC-BY license. Not internally tested by R&D Systems.
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Detection of Human G9a/EHMT2 by Immunohistochemistry G9a expression is positively correlated with cell proliferation and poor prognosis in HNSCC patients. (A) Correlation between Ki-67 and G9a expression. The expression of two proteins were analyzed by IHC staining of tissue microarray (TMA) containing 46 normal and 108 tumor sections from HNSCC patients. The expression level of both Ki-67 and G9a was measured by relative staining intensity multiplied by percentage. Scale bar, 50 μm. (*, p < 0.05; ***, p < 0.001). (B) Ki-67 and G9a IHC staining of serial section of tumors. Low or high Ki-67 and G9a expression were defined by a threshold of 80 and 143, which is the average of relative staining intensity multiplied by percentage of Ki-67 and G9a. Scale bar, 50 μm. (***, p < 0.001). (C) Kaplan-Meier plot of overall survival of 77 HNSCC patients from a National Taiwan University Hospital cohort stratified by G9a expression level. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25027955), licensed under a CC-BY license. Not internally tested by R&D Systems.
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Detection of Human G9a/EHMT2 by Immunohistochemistry G9a expression is positively correlated with cell proliferation and poor prognosis in HNSCC patients. (A) Correlation between Ki-67 and G9a expression. The expression of two proteins were analyzed by IHC staining of tissue microarray (TMA) containing 46 normal and 108 tumor sections from HNSCC patients. The expression level of both Ki-67 and G9a was measured by relative staining intensity multiplied by percentage. Scale bar, 50 μm. (*, p < 0.05; ***, p < 0.001). (B) Ki-67 and G9a IHC staining of serial section of tumors. Low or high Ki-67 and G9a expression were defined by a threshold of 80 and 143, which is the average of relative staining intensity multiplied by percentage of Ki-67 and G9a. Scale bar, 50 μm. (***, p < 0.001). (C) Kaplan-Meier plot of overall survival of 77 HNSCC patients from a National Taiwan University Hospital cohort stratified by G9a expression level. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25027955), licensed under a CC-BY license. Not internally tested by R&D Systems.
Preparation and Storage
Background: G9a/EHMT2
Histone H3 Lys 9 (H3-K9) methylation is a crucial epigenetic marker for transcriptional silencing. G9a is the major mammalian H3-K9 methyltransferase that targets euchromatic regions and is essential for mouse embryogenesis.
Product Datasheets
Citations for Human/Mouse G9a/EHMT2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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SMCHD1 maintains heterochromatin and genome compartments in human myoblasts
Authors: Huang, Z;Cui, W;Ratnayake, I;Tawil, R;Pfeifer, GP;
bioRxiv : the preprint server for biology
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
High G9a Expression in DLBCL and Its Inhibition by Niclosamide to Induce Autophagy as a Therapeutic Approach
Authors: Hsu, CM;Chang, KC;Chuang, TM;Chu, ML;Lin, PW;Liu, HS;Kao, SY;Liu, YC;Huang, CT;Wang, MH;Yeh, TJ;Gau, YC;Du, JS;Wang, HC;Cho, SF;Hsiao, CE;Tsai, Y;Hsiao, SY;Hung, LC;Yen, CH;Hsiao, HH;
Cancers
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
TNF? Effects on Adipocytes Are Influenced by the Presence of Lysine Methyltransferases, G9a (EHMT2) and GLP (EHMT1)
Authors: Able, AA;Richard, AJ;Stephens, JM;
Biology
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Histone Methyltransferase G9a-Promoted Progression of Hepatocellular Carcinoma Is Targeted by Liver-Specific Hsa-miR-122
Authors: LT Yuan, WJ Lee, YC Yang, BR Chen, CY Yang, MW Chen, JQ Chen, M Hsiao, MH Chien, KT Hua
Cancers, 2021-05-14;13(10):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Inhibition of histone methyltransferase G9a attenuates liver cancer initiation by sensitizing DNA-damaged hepatocytes to p53-induced apoptosis
Authors: T Nakatsuka, K Tateishi, H Kato, H Fujiwara, K Yamamoto, Y Kudo, H Nakagawa, Y Tanaka, H Ijichi, T Ikenoue, T Ishizawa, K Hasegawa, M Tachibana, Y Shinkai, K Koike
Cell Death & Disease, 2021-01-19;12(1):99.
Species: Mouse
Sample Types: Tissue Homogenates, Whole Tissue
Applications: IHC, Western Blot -
EWSR1 affects PRDM9-dependent histone 3 methylation and provides a link between recombination hotspots and the chromosome axis
Authors: Tian H, Billings T, Walker M et al.
Mol Biol Cell
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Cyclin D1 integrates G9a-mediated histone methylation
Authors: Z Li, X Jiao, G Di Sante, A Ertel, MC Casimiro, M Wang, S Katiyar, X Ju, DV Klopfenste, A Tozeren, W Dampier, I Chepelev, A Jeltsch, RG Pestell
Oncogene, 2019-02-04;0(0):.
Species: Mouse
Sample Types: Cell Lysates, Whole Cells
Applications: ChIP, ICC, Western Blot -
Mutual inhibition between HDAC9 and miR-17 regulates osteogenesis of human periodontal ligament stem cells in inflammatory conditions
Authors: L Li, W Liu, H Wang, Q Yang, L Zhang, F Jin, Y Jin
Cell Death Dis, 2018-05-01;9(5):480.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Methyltransferase G9a promotes cervical cancer angiogenesis and decreases patient survival
Authors: RJ Chen, CT Shun, ML Yen, CH Chou, MC Lin
Oncotarget, 2017-07-07;8(37):62081-62098.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Epigenetic therapy with inhibitors of histone methylation suppresses DNA damage signaling and increases glioma cell radiosensitivity
Authors: Ozge Gursoy-Yuzugullu, Chelsea Carman, Rodolfo Bortolozo Serafim, Marios Myronakis, Valeria Valente, Brendan D. Price
Oncotarget
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Histone methyltransferase G9a drives chemotherapy resistance by regulating the glutamate-cysteine ligase catalytic subunit in head and neck squamous cell carcinoma
Authors: CW Liu, KT Hua, KC Li, HF Kao, RL Hong, JY Ko, M Hsiao, ML Kuo, CT Tan
Mol. Cancer Ther, 2017-03-06;0(0):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Epigenetic response to environmental stress: Assembly of BRG1–G9a/GLP–DNMT3 repressive chromatin complex on Myh6 promoter in pathologically stressed hearts☆,☆☆
Authors: Pei Han, Wei Li, Jin Yang, Ching Shang, Chiou-Hong Lin, Wei Cheng et al.
Biochimica et Biophysica Acta (BBA) - Molecular Cell Research
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EHMT2 directs DNA methylation for efficient gene silencing in mouse embryos
Authors: Ghislain Auclair, Julie Borgel, Lionel A. Sanz, Judith Vallet, Sylvain Guibert, Michael Dumas et al.
Genome Research
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Inhibition of G9a induces DUSP4-dependent autophagic cell death in head and neck squamous cell carcinoma.
Authors: Li, Kai-Chun, Hua, Kuo-Tai, Lin, Yi-Shen, Su, Chia-Yi, Ko, Jenq-Yuh, Hsiao, Michael, Kuo, Min-Lian, Tan, Ching-Ti
Mol Cancer, 2014-07-15;13(0):172.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
The methyltransferase G9a regulates HoxA9-dependent transcription in AML.
Authors: Lehnertz B, Pabst C, Su L, Miller M, Liu F, Yi L, Zhang R, Krosl J, Yung E, Kirschner J, Rosten P, Underhill T, Jin J, Hebert J, Sauvageau G, Humphries R, Rossi F
Genes Dev, 2014-02-15;28(4):317-27.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
H3K9 histone methyltransferase G9a promotes lung cancer invasion and metastasis by silencing the cell adhesion molecule Ep-CAM.
Authors: Chen MW, Hua KT, Kao HJ, Chi CC, Wei LH, Johansson G, Shiah SG, Chen PS, Jeng YM, Cheng TY, Lai TC, Chang JS, Jan YH, Chien MH, Yang CJ, Huang MS, Hsiao M, Kuo ML
Cancer Res., 2010-10-12;70(20):7830-40.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
DNA methylation in ES cells requires the lysine methyltransferase G9a but not its catalytic activity.
Authors: Dong K, Maksakova I, Mohn F, Leung D, Appanah R, Lee S, Yang H, Lam L, Mager D, Schubeler D, Tachibana M, Shinkai Y, Lorincz M
EMBO J, 2008-09-25;27(20):2691-701.
Species: Mouse
Sample Types: Protein
Applications: Western Blot
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