Human/Mouse/Rat beta -Actin Antibody Summary
Accession # P60709
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
Detection of Human, Mouse, and Rat beta ‑Actin by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line, C2C12 mouse myoblast cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.01 µg/mL of Mouse Anti-Human/Mouse/Rat beta -Actin Monoclonal Antibody (Catalog # MAB8929) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for beta -Actin at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
beta ‑Actin in NIH‑3T3 Mouse Cell Line. beta -Actin was detected in immersion fixed NIH-3T3 mouse embryonic fibroblast cell line using Mouse Anti-Human/Mouse/Rat beta -Actin Monoclonal Antibody (Catalog # MAB8929) at 0.1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Cells were fixated in methanol. Specific staining was localized to cytoskeleton.
Detection of Human beta ‑Actin by Simple WesternTM. Simple Western lane view shows lysate of A431 human epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for beta ‑Actin at approximately 49 kDa (as indicated) using 1 µg/mL of Mouse Anti-Human/Mouse/Rat beta ‑Actin Monoclonal Antibody (Catalog # MAB8929). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Detection of Human, Mouse, and Rat beta ‑Actin by Simple WesternTM. Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, HeLa human cervical epithelial carcinoma cell line, C2C12 mouse myoblast cell line, and C6 rat glioma cell line, loaded at 0.2 mg/mL. A specific band was detected for beta ‑Actin at approximately 48 kDa (as indicated) using 1 µg/mL of Mouse Anti-Human/Mouse/Rat beta ‑Actin Monoclonal Antibody (Catalog # MAB8929). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Detection of Human beta-Actin by Western Blot The treatment of n-BP rescued the progression of QA-induced excitotoxicity in SCA3 PPs. The control and/or SCA3 PPs were treated with or without QA (1 μM) in the presence of n-BP (0, 10, 20, and 40 μg/mL) for 12 h. (A) Images of QA-treated SCA3 PPs in the presence of BP (40 μg/mL). (B) Confocal images of poly Q localization in BP (40 μg/mL)-treated SCA3 PPs in the presence of QA (1 um). When compared with Figure 2C, 40 μg/mL of n-BP prevented the amounts of colocalized polyQ in the cell nucleus of PPs. The distribution of polyQ in the nucleus is indicated by white arrowheads. Scale bar = 100 μm; (C) protein analysis of wild-type, mutant ATXN3 and its proteolytic fragment in cell lysates of control and SCA3 PPs, as assessed by immunoblots. Soluble, soluble protein; Insoluble, insoluble protein; (D,E) representative immunoblot of control and cleaved PARP1 in cell lysates of control and SCA3 PPs, as assessed by immunoblots. The HDAC2 were used as loading controls. The quantification from three independent images is presented as the means ± standard deviation. t(4) = 2.824, p < 0.05, for lane 1 vs. lane 2; t(4) = 5.613, p < 0.01, for lane 1 vs. lane 3; t(4) = 3.522, p < 0.05, for lane 1 vs. lane 4; (F) the quantification result of calcium concentration in SCA3 PPs, as assessed by Fura-2 indicator. Cells were treated with QA (1 μM) in the presence of n-BP (0, 10, 20, and 40 μg/mL). Data are presented as the means ± standard deviation. t(4) = 2.95, p < 0.05; (G) the calpain activity in control and SCA3 PPs cell lysates, as assessed by ELISA assay. The quantification results from three independent replicates are presented as the means ± standard deviation. t(4) = 3.27, p < 0.05, for lane 3 vs. lane 4; t(4) = 17.55, p < 0.01, for lane 3 vs. lane 5; t(4) = 9.019, p < 0.01, for lane 3 vs. lane 6. (H) Protein analysis of calpain 1, calpain 2 and calpastatin in cell lysates of SCA3 PPs, as assessed by immunoblots. Cells were treated with or without QA (1 μM) in the presence of n-BP (0, 10, 20, and 40 μg/mL). *, p < 0.05; **, p < 0.01. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/35163312), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human beta-Actin by Simple Western Identification of menin-associated proteins (MAPs) in breast cancer cells. (A) and (B) WES of BirA-Menin fusion proteins (A) and biotin-labeled proteins (B) in total lysates of BirA-MEN1 BioID engineered T47D or MCF-7 cells after incubating with or without doxycycline and biotin. (C) Schematic purification and proteomic identification of MAPs using LC–MS/MS. (D) Heatmap of the quantification of 35 MAPs commonly shared in T47D and MCF-7 cells. MAPs further verified by WES immunoassays were indicated by arrows. (E) Network analysis of 35 MAPs in MCF-7 cells. The distance between menin and MAPs represented the quantitative ratio of each MAP and menin. MAPs marked in blue were further assayed by WES. (F) Nuclear or cytoplasmic lysates of BirA-MEN1 BioID engineered T47D or MCF-7 cells after streptavidin beads pull-down were detected by WES with antibodies against menin, KMT2A, MED12, WAPL, GATA3, LaminA/C, or GAPDH. FL, full length; SP, spliced form. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32971831), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human beta-Actin by Simple Western Identification of MEN1-modulated genes in breast cancer cells. (A) RT-qPCR of MEN1 in T47D or MCF-7 cells treated with vehicle or MEN1 shRNA lentivirus (n = 3). (B) Quantitative Western immunoassays (WES) of menin expression in T47D or MCF-7 cells treated with vehicle or MEN1 shRNA lentivirus (n = 3). (C) Venn diagrams of differentially expressed genes (fold change ≥1.5 or ≤0.66) in T47D or MCF-7 cells after shMEN1 knockdown compared with vehicle controls (n = 2). (D) Pathway annotation analysis of MEN1-upregulated and MEN1-downregulated genes in T47D or MCF-7 cells using DAVID including cancer hallmark pathways. (E) Schematic illustration of five major metabolic pathways. (F) Expression heat maps of oxidative phosphorylation (OXPHOS) and glycolytic genes in both MEN1 knockdown T47D and MCF-7 cells (fold changes relative to vehicle controls). (G) Bar charts of the expression levels of representative OXPHOS and glycolytic genes affected by MEN1 knockdown in T47D or MCF-7 cells using RT-qPCR. Data are presented as mean ± S.D. Unpaired two-tailed Student’s t-test was used for statistics. * p < 0.05, ** p < 0.01, and *** p < 0.001. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32971831), licensed under a CC-BY license. Not internally tested by R&D Systems.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: beta-Actin
Actin makes
up the most abundant protein in many eukaryotic cell types. It polymerizes to
form microfilaments that have an array of functions, including regulating
contractility, motility, cytokinesis, phagocytosis, adhesion, cell morphology,
and providing structural support. At least six types of Actin, falling into
three categories, exist in higher eukaryotes. The three types, separated based on
isoelectric point, include alpha ( alpha
-Skeletal, alpha -Cardiac, alpha -Smooth
Muscle), beta ( beta -Non-muscle) and gamma
( gamma -Smooth Muscle, gamma -non-muscle) isoforms. Non-muscle
beta - and gamma -actin are also known as cytoplasmic actin.
Product Datasheets
Citations for Human/Mouse/Rat beta -Actin Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Correlation between the rs7101 and rs1063169 polymorphisms in the FOS noncoding region and susceptibility to and prognosis of colorectal cancer
Authors: Hongshu Chen, Lijuan Ji, Xiuzhen Liu, Jihong Zhong
Medicine (Baltimore)
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Histone deacetylase�5 promotes the proliferation and invasion of lung cancer cells
Authors: L Zhong, S Sun, S Yao, X Han, M Gu, J Shi
Oncol. Rep., 2018-07-24;0(0):.
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Salvia miltiorrhiza Injection Alleviates LPS-Induced Acute Lung Injury by Adjusting the Balance of MMPs/TIMPs Ratio
Authors: Chen G, Ge D, Zhu B et al.
Evidence-Based Complementary and Alternative Medicine
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High-Fat-Diet Suppressed Ketone Body Utilization for Lipogenic Pathway in Brown Adipose Tissues
Authors: M Yamasaki, S Hasegawa, S Ozaki, M Imai, D Saito, N Takahashi
Metabolites, 2023-04-05;13(4):.
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Plant extract from Caesalpinia spinosa inhibits cancer-associated fibroblast-like cells generation and function in a tumor microenvironment model
Authors: MC Jiménez, K Prieto, P Lasso, M Gutiérrez, V Rodriguez-, S Fiorentino, A Barreto
Heliyon, 2023-03-02;9(3):e14148.
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Triple negative breast cancer migration is modified by mitochondrial metabolism alteration induced by natural extracts of C. spinosa and P. alliacea
Authors: Carlosama, C;Arévalo, C;Jimenez, MC;Lasso, P;Urueña, C;Fiorentino, S;Barreto, A;
Scientific reports
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
PAI-1 uncouples integrin-?1 from restrain by membrane-bound ?-catenin to promote collagen fibril remodeling in obesity-related neoplasms
Authors: Lin, LL;Nayak, B;Osmulski, PA;Wang, E;Wang, CP;Valente, PT;Wang, CM;Tan, X;Santanam, N;Wang, TL;Gaczynska, ME;Kost, ER;Huang, TH;Kirma, NB;
Cell reports
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Bioinformatics Analysis of Human Papillomavirus 16 Integration in Cervical Cancer: Changes in MAGI-1 Expression in Premalignant Lesions and Invasive Carcinoma
Authors: Catalán-Castorena, O;Garibay-Cerdenares, OL;Illades-Aguiar, B;Castillo-Sánchez, R;Zubillaga-Guerrero, MI;Leyva-Vazquez, MA;Encarnacion-Guevara, S;Flores-Alfaro, E;Ramirez-Ruano, M;Del Carmen Alarcón-Romero, L;
Cancers
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
The extracellular niche and tumor microenvironment enhance KRAS inhibitor efficacy in pancreatic cancer
Authors: Kumarasamy, V;Wang, J;Frangou, C;Wan, Y;Dynka, A;Rosenheck, H;Dey, P;Abel, EV;Knudsen, ES;Witkiewicz, AK;
Cancer research
Species: Human, Mouse
Sample Types: Whole Cells
Applications: Western Blot -
Unveiling the potent effect of vitamin D: harnessing Nrf2/HO-1 signaling pathways as molecular targets to alleviate urban particulate matter-induced asthma inflammation
Authors: Ge, D;Chen, Q;Xie, X;Li, Q;Yang, Y;
BMC pulmonary medicine
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Glutathione Reductase Expression and Its Prognostic Significance in Colon Cancer
Authors: Brzozowa-Zasada, M;Piecuch, A;Bajdak-Rusinek, K;Michalski, M;Klymenko, O;Matysiak, N;Janelt, K;Czuba, Z;
International journal of molecular sciences
Species: Human
Sample Types: Protein
Applications: Western Blot -
Plant extracts modulate cellular stress to inhibit replication of mouse Coronavirus MHV-A59
Authors: Prieto, K;Arévalo, C;Lasso, P;Carlosama, C;Urueña, C;Fiorentino, S;Barreto, A;
Heliyon
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
A Prognostic Activity of Glutaredoxin 1 Protein (Grx1) in Colon Cancer
Authors: Brzozowa-Zasada, M;Piecuch, A;Bajdak-Rusinek, K;Go??bek, K;Michalski, M;Matysiak, N;Czuba, Z;
International journal of molecular sciences
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Co-targeting HSP90 alpha and CDK7 overcomes resistance against HSP90 inhibitors in BCR-ABL1+ leukemia cells
Authors: Vogt, M;Dienstbier, N;Schliehe-Diecks, J;Scharov, K;Tu, JW;Gebing, P;Hogenkamp, J;Bilen, BS;Furlan, S;Picard, D;Remke, M;Yasin, L;Bickel, D;Kalia, M;Iacoangeli, A;Lenz, T;Stühler, K;Pandyra, AA;Hauer, J;Fischer, U;Wagener, R;Borkhardt, A;Bhatia, S;
Cell death & disease
Species: N/A
Sample Types: Recombinant Protein
Applications: Western Blot -
A?1-42 Accumulation Accompanies Changed Expression of Ly6/uPAR Proteins, Dysregulation of the Cholinergic System, and Degeneration of Astrocytes in the Cerebellum of Mouse Model of Early Alzheimer Disease
Authors: Bychkov, ML;Isaev, AB;Andreev-Andrievskiy, AA;Petrov, K;Paramonov, AS;Kirpichnikov, MP;Lyukmanova, EN;
International journal of molecular sciences
Species: Transgenic Mouse
Sample Types: Protein
Applications: Western Blot -
Copper infused fabric attenuates inflammation in macrophages
Authors: Zangiabadi, S;Chamoun, KP;Nguyen, K;Tang, Y;Sweeney, G;Abdul-Sater, AA;
PloS one
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Exploring the impact of dexamethasone on gene regulation in myeloma cells
Authors: Victor Bessonneau-Gaborit, Jonathan Cruard, Catherine Guerin-Charbonnel, Jennifer Derrien, Jean-Baptiste Alberge, Elise Douillard et al.
Life Science Alliance
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Macrophages Provide Essential Support for Erythropoiesis, and Extracellular ATP Contributes to a Erythropoiesis-Supportive Microenvironment during Repeated Psychological Stress
Authors: Momčilović, S;Bogdanović, A;Milošević, MS;Mojsilović, S;Marković, DC;Kočović, DM;Vignjević Petrinović, S;
International journal of molecular sciences
Species: Mouse
Sample Types: Cell Lysates, Tissue Homogenates
Applications: Western Blot -
Development of the first geldanamycin-based HSP90 degraders
Authors: Silas Wurnig, Melina Vogt, Julian Hogenkamp, Niklas Dienstbier, Arndt Borkhardt, Sanil Bhatia et al.
Frontiers in Chemistry
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Gut microbiota mediated the therapeutic efficiency of Simiao decoction in the treatment of gout arthritis mice
Authors: Lin, X;Wang, M;He, Z;Hao, G;
BMC complementary medicine and therapies
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Immunohistochemical Expression of Glutathione Peroxidase 1 (Gpx-1) as an Independent Prognostic Factor in Colon Adenocarcinoma Patients
Authors: Marlena Brzozowa-Zasada, Adam Piecuch, Karolina Bajdak-Rusinek, Kamil Janelt, Marek Michalski, Olesya Klymenko et al.
Pharmaceuticals (Basel)
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Effect of stimulator of interferon genes (STING) signaling on radiation-induced chemokine expression in human osteosarcoma cells
Authors: SS Withers, CE Moeller, CN Quick, CC Liu, SM Baham, JS Looper, R Subramania, KG Kousoulas
PLoS ONE, 2023-04-20;18(4):e0284645.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Pharmacologically targeting KRAS G12D in PDAC models: tumor cell intrinsic and extrinsic impact
Authors: Kumarasamy, V;Frangou, C;Wang, J;Wan, Y;Dynka, A;Rosenheck, H;Dey, P;Abel, EV;Knudsen, ES;Witkiewicz, AK;
bioRxiv : the preprint server for biology
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Shotgun Proteomics of Co-Cultured Leukemic and Bone Marrow Stromal Cells from Different Species as a Preliminary Approach to Detect Intercellular Protein Transfer
Authors: AJ Nevárez-Ra, AL Guzmán-Ort, P Cortes-Rey, E Perez-Sala, GA Jaimes-Ort, R Valle-Rios, Á Marín-Hern, JS Rodríguez-, E Ruiz-May, JL Castrejón-, H Quezada
Proteomes, 2023-04-05;11(2):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Metformin induces pyroptosis in leptin receptor-defective hepatocytes via overactivation of the AMPK axis
Authors: B Liu, J Xu, L Lu, L Gao, S Zhu, Y Sui, T Cao, T Yang
Cell Death & Disease, 2023-02-03;14(2):82.
Species: Rat
Sample Types: Cell Lysates
Applications: Western Blot -
Altered host protease determinants for SARS-CoV-2 Omicron
Authors: JF Chan, X Huang, B Hu, Y Chai, H Shi, T Zhu, TT Yuen, Y Liu, H Liu, J Shi, L Wen, H Shuai, Y Hou, C Yoon, JP Cai, AJ Zhang, J Zhou, F Yin, S Yuan, BZ Zhang, MA Brindley, ZL Shi, KY Yuen, H Chu
Science Advances, 2023-01-20;9(3):eadd3867.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Effect of stimulator of interferon genes (STING) signaling on radiation-induced chemokine expression in human osteosarcoma cells
Authors: SS Withers, CE Moeller, CN Quick, CC Liu, SM Baham, JS Looper, R Subramania, KG Kousoulas
PLoS ONE, 2023;18(4):e0284645.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Human Umbilical Cord Mesenchymal Stem Cells Attenuate Severe Burn-Induced Multiple Organ Injury via Potentiating IGF-1 and BCL-2/BAX Pathway
Authors: H Wang, T Ba, Q Wang, L Yang, C Li, X Hao, Y Yin, L Liu
Stem Cells International, 2022-12-23;2022(0):5474289.
Species: Rat
Sample Types: Tissue Lysates
Applications: Western Blot -
Anastellin impacts on the processing of extracellular matrix fibronectin and stimulates release of cytokines from coronary artery smooth muscle cells
Authors: J He, JH Steffen, PW Thulstrup, JN Pedersen, MB Sauerland, DE Otzen, CL Hawkins, P Gourdon, MJ Davies, P Hägglund
Scientific Reports, 2022-12-21;12(1):22051.
Species: Human
Sample Types: Protein Lysate
Applications: Western Blot -
Low-energy shock wave therapy ameliorates ischemic-induced overactive bladder in a rat model
Authors: S Kimura, N Kawamorita, Y Kikuchi, T Shindo, Y Ishizuka, Y Satake, T Sato, H Izumi, S Yamashita, S Yasuda, H Shimokawa, A Ito
Scientific Reports, 2022-12-19;12(1):21960.
Species: Rat
Sample Types: Protein
Applications: Western Blot -
Deregulated RNAs involved in sympathetic regulation of sepsis-induced acute lung injury based on whole transcriptome sequencing
Authors: J Zhang, Z Zhang, X Nie, Y Liu, Y Qi, J Wang
BMC Genomics, 2022-12-16;23(1):836.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Rapid Generation of Circulating and Mucosal Decoy Human ACE2 using mRNA Nanotherapeutics for the Potential Treatment of SARS‐CoV‐2
Authors: Jeonghwan Kim, Antony Jozic, Anindit Mukherjee, Dylan Nelson, Kevin Chiem, Md Siddiqur Rahman Khan et al.
Advanced Science
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Extracellular vesicles carrying HIV-1 Nef induce long-term hyperreactivity of myeloid cells
Authors: L Dubrovsky, B Brichacek, NM Prashant, T Pushkarsky, N Mukhamedov, AJ Fleetwood, Y Xu, D Dragoljevi, M Fitzgerald, A Horvath, AJ Murphy, D Sviridov, MI Bukrinsky
Cell Reports, 2022-11-22;41(8):111674.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Novel fat taste receptor agonists curtail progressive weight gain in obese male mice
Authors: AS Khan, A Hichami, B Murtaza, ML Louillat-H, C Ramseyer, M Azadi, S Yesylevsky, F Mangin, F Lirussi, J Leemput, JF Merlin, A Schmitt, M Suliman, J Bayardon, S Semnanian, S Jugé, NA Khan
Cellular and Molecular Gastroenterology and Hepatology, 2022-11-19;0(0):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Exosome Release by Glucose Deprivation Is Important for the Viability of TSC-Null Cells
Authors: JH Bae, JH Kim
Cells, 2022-09-14;11(18):.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
CAPG Is Required for Ebola Virus Infection by Controlling Virus Egress from Infected Cells
Authors: Hiroyuki Mori, James P. Connell, Callie J. Donahue, RuthMabel Boytz, Yen Thi Kim Nguyen, Daisy W. Leung et al.
Viruses
Applications: Simple Western -
Cinnamaldehyde and Curcumin Prime Akt2 for Insulin-Stimulated Activation
Authors: Y Urasaki, TT Le
Nutrients, 2022-08-12;14(16):.
Species: Human
Sample Types: Whole Cells
Applications: Simple Western -
TRIM7 Restricts Coxsackievirus and Norovirus Infection by Detecting the C-Terminal Glutamine Generated by 3C Protease Processing
Authors: Jakub Luptak, Donna L. Mallery, Aminu S. Jahun, Anna Albecka, Dean Clift, Osaid Ather et al.
Viruses
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Intranasal immunization with avian paramyxovirus type 3 expressing SARS-CoV-2 spike protein protects hamsters against SARS-CoV-2
Authors: Hong-Su Park, Yumiko Matsuoka, Cindy Luongo, Lijuan Yang, Celia Santos, Xueqiao Liu et al.
npj Vaccines
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Development of a First-in-Class Small-Molecule Inhibitor of the C-Terminal Hsp90 Dimerization
Authors: Sanil Bhatia, Lukas Spanier, David Bickel, Niklas Dienstbier, Vitalij Woloschin, Melina Vogt et al.
ACS Central Science
Applications: Simple Western -
Soy Phospholipids Exert a Renoprotective Effect by Inhibiting the Nuclear Factor Kappa B Pathway in Macrophages
Authors: S Ohta, M Asanoma, N Irie, N Tachibana, M Kohno
Metabolites, 2022-04-06;12(4):.
Species: Human
Sample Types: Cell Lysates
Applications: Simple Western -
A VDAC1-mediated NEET protein chain transfers [2Fe-2S] clusters between the mitochondria and the cytosol and impacts mitochondrial dynamics
Authors: O Karmi, HB Marjault, F Bai, S Roy, YS Sohn, M Darash Yah, F Morcos, K Ioannidis, Y Nahmias, PA Jennings, R Mittler, JN Onuchic, R Nechushtai
Proceedings of the National Academy of Sciences of the United States of America, 2022-02-15;119(7):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
The SDF1-CXCR4 Axis Is Involved in the Hyperbaric Oxygen Therapy-Mediated Neuronal Cells Migration in Transient Brain Ischemic Rats
Authors: Ray-Yau Wang, Yea-Ru Yang, Heng-Chih Chang
International Journal of Molecular Sciences
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Anagliptin prevents lipopolysaccharide (LPS)- induced inflammation and activation of macrophages
Authors: F Yu, W Tian, J Dong
International immunopharmacology, 2022-01-16;104(0):108514.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
2-Hydroxyglutarate destabilizes chromatin regulatory landscape and lineage fidelity to promote cellular heterogeneity
Authors: M Kusi, M Zand, LL Lin, M Chen, A Lopez, CL Lin, CM Wang, ND Lucio, NB Kirma, J Ruan, TH Huang, K Mitsuya
Cell Reports, 2022-01-11;38(2):110220.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Cyclo(Val-Pro) and Cyclo(Leu-Hydroxy-Pro) from Pseudomonas sp. (ABS-36) alleviates acute and chronic renal injury under in vitro and in vivo models (Ischemic reperfusion and unilateral ureter obstruction)
Authors: K Hira, P Sharma, A Mahale, O Prakash Ku, A Sajeli Beg
International immunopharmacology, 2021-12-29;103(0):108494.
Species: Mouse, Rat
Sample Types: Cell Lysates
Applications: Western Blot -
Enhanced interpretation of 935 hotspot and non-hotspot RAS variants using evidence-based structural bioinformatics
Authors: S Tripathi, NR Dsouza, AJ Mathison, E Leverence, R Urrutia, MT Zimmermann
Computational and structural biotechnology journal, 2021-12-11;20(0):117-127.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Investigating receptor-mediated antibody transcytosis using blood–brain barrier organoid arrays
Authors: Claire Simonneau, Martina Duschmalé, Alina Gavrilov, Nathalie Brandenberg, Sylke Hoehnel, Camilla Ceroni et al.
Fluids and Barriers of the CNS
Applications: Simple Western -
NFAT5/TonEBP Limits Pulmonary Vascular Resistance in the Hypoxic Lung by Controlling Mitochondrial Reactive Oxygen Species Generation in Arterial Smooth Muscle Cells
Authors: Laban H, Siegmund S, Zappe M et al.
Cells
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Nanotube‐like processes facilitate material transfer between photoreceptors
Authors: Aikaterini A Kalargyrou, Mark Basche, Aura Hare, Emma L West, Alexander J Smith, Robin R Ali et al.
EMBO reports
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Disrupting CISD2 function in cancer cells primarily impacts mitochondrial labile iron levels and triggers TXNIP expression
Authors: Karmi O, Sohn YS, Zandalinas SI et al.
Free Radical Biology and Medicine
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Cytochrome P450 3A4 (CYP3A4) protein quantification using capillary western blot technology and total protein normalization
Authors: JM Collins, D Wang
Journal of pharmacological and toxicological methods, 2021-08-30;112(0):107117.
Species: Human
Sample Types: Tissue Lysates
Applications: Simple Western -
Phosphorylation in the Charged Linker Modulates Interactions and Secretion of Hsp90beta
Authors: L Weidenauer, M Quadroni
Cells, 2021-07-05;10(7):.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Discovery of Small Anti‐ACE2 Peptides to Inhibit SARS‐CoV‐2 Infectivity
Authors: Pratik Adhikary, Sashi Kandel, Umar‐Farouk Mamani, Bahaa Mustafa, Siyuan Hao, Jianming Qiu et al.
Advanced Therapeutics
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Cyclooxygenase inhibition attenuates brain angiogenesis and independently decreases mouse survival under hypoxia
Authors: Drew R. Seeger, Svetlana A. Golovko, Bryon D. Grove, Mikhail Y. Golovko
Journal of Neurochemistry
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CEACAM6’s Role as a Chemoresistance and Prognostic Biomarker for Pancreatic Cancer: A Comparison of CEACAM6’s Diagnostic and Prognostic Capabilities with Those of CA19-9 and CEA
Authors: Benediktas Kurlinkus, Marija Ger, Algirdas Kaupinis, Eugenijus Jasiunas, Mindaugas Valius, Audrius Sileikis
Life (Basel)
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ACE2/ACE imbalance and impaired vasoreparative functions of stem/progenitor cells in aging
Authors: S. Joshi, K. Chittimalli, J. Jahan, G. Vasam, Y. P. Jarajapu
GeroScience
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Mesenchymal stromal cell mitochondrial transfer to human induced T-regulatory cells mediates FOXP3 stability
Authors: JS Do, D Zwick, JD Kenyon, F Zhong, D Askew, AY Huang, W Van't Hof, M Finney, MJ Laughlin
Scientific Reports, 2021-05-21;11(1):10676.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Spaceflight and hind limb unloading induces an arthritic phenotype in knee articular cartilage and menisci of rodents
Authors: AT Kwok, NS Mohamed, JF Plate, RR Yammani, S Rosas, TA Bateman, E Livingston, JE Moore, BA Kerr, J Lee, CM Furdui, L Tan, ML Bouxsein, VL Ferguson, LS Stodieck, DC Zawieja, MD Delp, XW Mao, JS Willey
Scientific Reports, 2021-05-18;11(1):10469.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
LCZ696 ameliorates lipopolysaccharide-induced endothelial injury
Authors: Aihong Gao, Yu Wang, Xiao Gao, Wei Tian
Aging (Albany NY)
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KEAP1 Is Required for Artesunate Anticancer Activity in Non-Small-Cell Lung Cancer
Authors: KS Hill, A McDowell, JR McCorkle, E Schuler, SR Ellingson, R Plattner, JM Kolesar
Cancers, 2021-04-14;13(8):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Development of cisplatin-loaded hydrogels for trans-portal vein chemoembolization in an orthotopic liver cancer mouse model
Authors: Xinxiang Yang, Wai-Ho Oscar Yeung, Kel Vin Tan, Tak-Pan Kevin Ng, Li Pang, Jie Zhou et al.
Drug Delivery
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ACE2 gene transfer ameliorates vasoreparative dysfunction in CD34+ cells derived from diabetic older adults
Authors: Shrinidh Joshi, Ildamaris Montes de Oca, Ahmad Maghrabi, Christine Lopez-Yang, Julio Quiroz-Olvera, Charles A. Garcia et al.
Clinical Science
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Host and viral determinants for efficient SARS-CoV-2 infection of the human lung
Authors: H Chu, B Hu, X Huang, Y Chai, D Zhou, Y Wang, H Shuai, D Yang, Y Hou, X Zhang, TT Yuen, JP Cai, AJ Zhang, J Zhou, S Yuan, KK To, IH Chan, KY Sit, DC Foo, IY Wong, AT Ng, TT Cheung, SY Law, WK Au, MA Brindley, Z Chen, KH Kok, JF Chan, KY Yuen
Nature Communications, 2021-01-08;12(1):134.
Species: Hamster - Mesocricetus auratus (Golden Hamster)
Sample Types: Cell Lysates
Applications: Western Blot -
Secreted protein acidic and rich in cysteine (SPARC) knockout mice have greater outflow facility
Authors: L Yu, Y Zheng, BJ Liu, MH Kang, JC Millar, DJ Rhee
PLoS ONE, 2020-11-04;15(11):e0241294.
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Effect of miR‑215 on the Expression of Tumor Suppressor Gene Rb1 in Retinoblastoma Cell Lines
Authors: Liqin SHAO, Zhangxing SHENG, Yuefeng ZHU, Jianchao LI, Rufa MENG
Iranian Journal of Public Health
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PAI-1-Dependent Inactivation of SMAD4-Modulated Junction and Adhesion Complex in Obese Endometrial Cancer
Authors: LL Lin, ER Kost, CL Lin, P Valente, CM Wang, MG Kolonin, AC Daquinag, X Tan, N Lucio, CN Hung, CP Wang, NB Kirma, TH Huang
Cell Rep, 2020-10-13;33(2):108253.
Species: Human
Sample Types: Cell Lysates
Applications: Simple Western -
Transplantation of Adipose-Derived Stem Cells Alleviates Striatal Degeneration in a Transgenic Mouse Model for Multiple System Atrophy
Authors: Christine Chang, Jen-Wei Liu, Bo Cheng Chen, Zhe Sheng Jiang, Chi Tang Tu, Che Hung Su et al.
Cell Transplant
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Menin and Menin-Associated Proteins Coregulate Cancer Energy Metabolism
Authors: CW Chou, X Tan, CN Hung, B Lieberman, M Chen, M Kusi, K Mitsuya, CL Lin, M Morita, Z Liu, CL Chen, TH Huang
Cancers (Basel), 2020-09-22;12(9):.
Species: Human
Sample Types: Cell Lysates
Applications: Immunoprecipitation, Western Blot -
Selenium Nanoparticles Pre-Treatment Reverse Behavioral, Oxidative Damage, Neuronal Loss and Neurochemical Alterations in Pentylenetetrazole-Induced Epileptic Seizures in Mice
Authors: X Yuan, Z Fu, P Ji, L Guo, AO Al-Ghamdy, A Alkandiri, OA Habotta, AE Abdel Mone, RB Kassab
Int J Nanomedicine, 2020-08-24;15(0):6339-6353.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
ASIC1a Inhibitor mambalgin-2 Suppresses the Growth of Leukemia Cells by Cell Cycle Arrest
Authors: M. L. Bychkov, M. A. Shulepko, V. Y. Vasileva, A. V. Sudarikova, M. P. Kirpichnikov, E. N. Lyukmanova
Acta Naturae
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Targeting QKI-7 in vivo restores endothelial cell function in diabetes
Authors: C Yang, M Eleftheria, S Kelaini, T Morrison, MV González, R Caines, N Edwards, A Yacoub, K Edgar, A Moez, A Ivetic, A Zampetaki, L Zeng, FL Wilkinson, N Lois, AW Stitt, DJ Grieve, A Margariti
Nat Commun, 2020-07-30;11(1):3812.
Species: Human, Mouse
Sample Types: Cell Lysates, Protein
Applications: Western Blot -
Cellular sensing of extracellular purine nucleosides triggers an innate IFN-&beta response
Authors: R Dhanwani, M Takahashi, IT Mathews, C Lenzi, A Romanov, JD Watrous, B Pieters, CC Hedrick, CA Benedict, J Linden, R Nilsson, M Jain, S Sharma
Sci Adv, 2020-07-22;6(30):eaba3688.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
A Cell-Based Target Engagement Assay for the Identification of Cereblon E3 Ubiquitin Ligase Ligands and Their Application in HDAC6 Degraders
Authors: Ka Yang, Yu Zhao, Xueqing Nie, Hao Wu, Bo Wang, Chelsi M. Almodovar-Rivera et al.
Cell Chemical Biology
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Simiao Decoction Alleviates Gouty Arthritis by Modulating Proinflammatory Cytokines and the Gut Ecosystem
Authors: X Lin, T Shao, L Huang, X Wen, M Wang, C Wen, Z He
Front Pharmacol, 2020-06-24;11(0):955.
Species: Mouse
Sample Types: Tissue Lysates
Applications: Western Blot -
Mitochondrial alterations triggered by repeated exposure to fine (PM2.5-0.18) and quasi-ultrafine (PM0.18) fractions of ambient particulate matter
Authors: J Sotty, J Kluza, C De Sousa, M Tardivel, S Anthérieu, LY Alleman, L Canivet, E Perdrix, A Loyens, P Marchetti, JM Lo Guidice, G Garçon
Environ Int, 2020-06-22;142(0):105830.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Hsa_circ_0085576 promotes clear cell renal cell carcinoma tumorigenesis and metastasis through the miR-498/YAP1 axis
Authors: Guanghua Liu, Jingmin Zhou, Yuanlin Piao, Xin Zhao, Yuzhi Zuo, Zhigang Ji
Aging (Albany NY)
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Effects of regulating miR-132 mediated GSK-3 beta on learning and memory function in mice
Authors: Gang Liu, Fengwei Yin, Congli Zhang, Yang Zhang, Xiaohong Li, Yunzhi Ling
Experimental and Therapeutic Medicine
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The critical role of MMP14 in adipose tissue remodeling during obesity
Authors: X Li, Y Zhao, C Chen, L Yang, HH Lee, Z Wang, N Zhang, MG Kolonin, Z An, X Ge, PE Scherer, K Sun
Mol. Cell. Biol., 2020-03-30;0(0):.
Species: Transgenic Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Activity of fibroblast-like synoviocytes in rheumatoid arthritis was impaired by dickkopf-1 targeting siRNA
Authors: YY Liu, SY Wang, YN Li, WJ Bian, LQ Zhang, YH Li, L Long, X Liu, XW Zhang, ZG Li
Chin. Med. J., 2020-03-20;0(6):679-686.
Species: Human
Sample Types: Cell Lysate
Applications: Western Blot -
Coenzyme Q10 Activates the Antioxidant Machinery and Inhibits the Inflammatory and Apoptotic Cascades Against Lead Acetate-Induced Renal Injury in Rats
Authors: Wafa A. AL-Megrin, Doaa Soliman, Rami B. Kassab, Dina M. Metwally, Ahmed E. Abdel Moneim, Manal F. El-Khadragy
Frontiers in Physiology
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Antagonistic Efficacy of Luteolin against Lead Acetate Exposure-Associated with Hepatotoxicity is Mediated via Antioxidant, Anti-Inflammatory, and Anti-Apoptotic Activities
Authors: Wafa A. AL-Megrin, Afrah F. Alkhuriji, Al Omar S. Yousef, Dina M. Metwally, Ola A. Habotta, Rami B. Kassab et al.
Antioxidants (Basel)
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Hypoxic regulation of angiotensin‐converting enzyme 2 and Mas receptor in human CD34+ cells
Authors: Shrinidh Joshi, Hannah Wollenzien, Estelle Leclerc, Yagna PR Jarajapu
Journal of Cellular Physiology
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Milk-derived exosomes (MDEs) have a different biological effect on�normal fetal colon epithelial cells compared to colon tumor cells in a miRNA-dependent manner
Authors: S Reif, Y Elbaum Shi, R Golan-Gers
J Transl Med, 2019-09-30;17(1):325.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Regulatory effect of miR‑144‑3p on the function of human trabecular meshwork cells and fibronectin‑1
Authors: Ruoxi Yin, Xiaoya Chen
Experimental and Therapeutic Medicine
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Royal jelly attenuates cadmium-induced nephrotoxicity in male mice
Authors: RS Almeer, GI AlBasher, S Alarifi, S Alkahtani, D Ali, AE Abdel Mone
Sci Rep, 2019-04-09;9(1):5825.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Enhanced Function of Induced Pluripotent Stem Cell-Derived Endothelial Cells Through ESM1 Signaling
Authors: Marta Vilà-González, Sophia Kelaini, Corey Magee, Rachel Caines, David Campbell, Magdalini Eleftheriadou et al.
Stem Cells
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A homozygous loss-of-function mutation leading to CYBC1 deficiency causes chronic granulomatous disease
Authors: GA Arnadottir, GL Norddahl, S Gudmundsdo, AB Agustsdott, S Sigurdsson, BO Jensson, K Bjarnadott, F Theodors, S Benonisdot, EV Ivarsdotti, A Oddsson, RP Kristjanss, G Sulem, KF Alexanders, T Juliusdott, KR Gudmundsso, J Saemundsdo, A Jonasdotti, A Jonasdotti, A Sigurdsson, P Manzanillo, SA Gudjonsson, GA Thorisson, OT Magnusson, G Masson, KB Orvar, H Holm, S Bjornsson, R Arngrimsso, DF Gudbjartss, U Thorsteins, I Jonsdottir, A Haraldsson, P Sulem, K Stefansson
Nat Commun, 2018-10-25;9(1):4447.
Species: Human
Sample Types: Whole Cells
Applications: Western Blot -
Enhancer Architecture and Essential Core Regulatory Circuitry of Chronic Lymphocytic Leukemia.
Authors: Ott C J, Federation A J et al.
Cancer Cell
Species: Human
Sample Types:
Applications: Simple Western -
ZO-1 protein is required for hydrogen peroxide to increase MDCK cell paracellular permeability in an ERK 1/2-dependent manner
Authors: Sahar Bilal, Shirin Jaggi, Danielle Janosevic, Nikita Shah, Shereen Teymour, Angelina Voronina et al.
American Journal of Physiology-Cell Physiology
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Bradykinin B2 receptor and dopamine D2 receptor cooperatively contribute to the regulation of neutrophil adhesion to endothelial cells*
Authors: Anna Niewiarowska-Sendo, Anna Łabędź-Masłowska, Andrzej Kozik, Ibeth Guevara-Lora
Acta Biochimica Polonica
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Oil-In-Water Microemulsions as Hosts for Benzothiophene-Based Cytotoxic Compounds: An Effective Combination
Authors: Ioanna Theochari, Vassiliki Papadimitriou, Demetris Papahatjis, Nikos Assimomytis, Efthimia Pappou, Harris Pratsinis et al.
Biomimetics (Basel)
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B-cell lymphoma 2 is associated with advanced tumor grade and clinical stage, and reduced overall survival in young Chinese patients with colorectal carcinoma
Authors: J Wang, G He, Q Yang, L Bai, B Jian, Q Li, Z Li
Oncol Lett, 2018-04-13;15(6):9009-9016.
Species: Human
Sample Types: Tissue Homogenates
Applications: Western Blot -
Association between circadian gene CLOCK and cisplatin resistance in ovarian cancer cells: A preliminary study
Authors: H Xu, Z Wang, G Mo, H Chen
Oncol Lett, 2018-04-13;15(6):8945-8950.
Species: Human
Sample Types: Whole Cells
Applications: Western Blot -
DNA methylation affects metastasis of renal cancer and is associated with TGF-?/RUNX3 inhibition
Authors: J Zheng, Y Mei, P Xiang, G Zhai, N Zhao, C Xu, M Liu, Z Pan, K Tang, D Jia
Cancer Cell Int., 2018-04-10;18(0):56.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Memantine protects against ischemia/reperfusion-induced brain endothelial permeability
Authors: Y Liu, Y Huang, Y Xu, P Qu, M Wang
IUBMB Life, 2018-03-15;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
TGF‑ beta 1 mediates the effects of aspirin on colonic tumor cell proliferation and apoptosis
Authors: Yuyi Wang, Chi Du, Nan Zhang, Mei Li, Yanyang Liu, Maoyuan Zhao et al.
Oncology Letters
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LncRNA TP73-AS1 Promotes Cell Proliferation and Inhibits Cell Apoptosis in Clear Cell Renal Cell Carcinoma Through Repressing KISS1 Expression and Inactivation of PI3K/Akt/mTOR Signaling Pathway
Authors: Guanghua Liu, Xin Zhao, Jingmin Zhou, Xiangming Cheng, Zixing Ye, Zhigang Ji
Cellular Physiology and Biochemistry
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Anti-neoplastic drugs increase caveolin-1-dependent migration, invasion and metastasis of cancer cells
Authors: NI Díaz-Valdi, CC Calderón, JE Díaz, L Lobos-Gonz, H Sepulveda, RJ Ortíz, S Martinez, V Silva, HJ Maldonado, P Silva, S Wehinger, VA Burzio, VA Torres, M Montecino, L Leyton, AFG Quest
Oncotarget, 2017-12-05;8(67):111943-111965.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Pharmacological inhibition of macrophage toll-like receptor 4/nuclear factor-kappa B alleviates rhabdomyolysis-induced acute kidney injury
Authors: RS Huang, JJ Zhou, YY Feng, M Shi, F Guo, SJ Gou, S Salerno, L Ma, P Fu
Chin. Med. J., 2017-09-20;0(0):.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Hochu-ekki-to Treatment Improves Reproductive and Immune Modulation in the Stress-Induced Rat Model of Polycystic Ovarian Syndrome
Authors: E Park, CW Choi, SJ Kim, YI Kim, S Sin, JP Chu, JY Heo
Molecules, 2017-06-13;22(6):.
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western Blot -
Cytoprotective Effects and Mechanisms of ?-17 Fatty Acid Desaturase in Injured Human Umbilical Vein Endothelial Cells (HUVECs)
Authors: H Zhou, C Wang
Med. Sci. Monit., 2017-04-04;23(0):1627-1635.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Microgravity Induction of TRAIL Expression in Preosteoclast Cells Enhances Osteoclast Differentiation
Sci Rep, 2016-05-04;6(0):25143.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Fatty acid-binding protein E-FABP restricts tumor growth by promoting IFN-beta responses in tumor-associated macrophages.
Authors: Zhang Y, Sun Y, Rao E, Yan F, Li Q, Zhang Y, Silverstein K, Liu S, Sauter E, Cleary M, Li B
Cancer Res, 2014-04-08;74(11):2986-98.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Crosstalk between monocytes and renal mesangial cells via interaction of metalloproteinases and fractalkine in diabetic nephropathy.
Authors: Wang Y, Wei Q, Liu Q, Li Z, Zhou L, Zou F, Yuan Y, Sun Z
Mol Med Rep, 2013-09-25;8(6):1817-23.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Receptor Interacting Protein Kinase Pathways Regulate Innate B Cell Developmental Checkpoints But Not Effector Function in Mice
Authors: Raksha Parthasarathy, Thomas Hägglöf, Jason T. Hadley, Alexandra McLennan, Aiden Mattke, Elizabeth A. Dudley et al.
Frontiers in Immunology
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Influence of bradykinin B2 receptor and dopamine D2 receptor on the oxidative stress, inflammatory response, and apoptotic process in human endothelial cells
Authors: A Niewiarows, A Kozik, I Guevara-Lo
PLoS ONE, 2018-11-14;13(11):e0206443.
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a-1,3-Fucosyltransferase-VII siRNA inhibits the expression of SLex and hepatocarcinoma cell proliferation.
Authors: Li D, Sun H, Bai G et al.
Int. J. Mol. Med.
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Inflammation induces stress erythropoiesis through heme-dependent activation of SPI-C.
Authors: Bennett L F, Liao C et al.
Sci Signal
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Mechanism of intrinsic resistance of lung squamous cell carcinoma to epithelial growth factor receptor‑tyrosine kinase inhibitors revealed by high‑throughput RNA interference screening
Authors: Lixia Ju, Zhiyi Dong, Juan Yang, Minghua Li
Oncology Letters
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Interleukin-1 Induces the Release of Lubricating Phospholipids from Human Osteoarthritic Fibroblast-like Synoviocytes
Authors: Thottakkattumana Parameswaran, V;Hild, C;Eichner, G;Ishaque, B;Rickert, M;Steinmeyer, J;
International journal of molecular sciences
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Used at 50 ng/ml
Antibody dilution was 1:100. Beta-Actin clearly expressed in mouse meniscus lysates, detected with ECL kit.
Actin expression in reconstructed human epidermis lysate (200 µg/mL protein concentration) detected in chemiluminescence.
Antibody dilution 1:50, works also at 1:100 dilution.
I have used for detecting expression of protein in Human and mouse Endothelial Progenitor cells (EPCs).