Human/Mouse/Rat FABP2/I-FABP Antibody

Catalog #: MAB30782 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB30782-100
MAB30782-SP

Detection of Human, Mouse, and Rat FABP2/I-FABP by Western Blot.

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Human/Mouse/Rat FABP2/I-FABP Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human FABP2/I-FABP in direct ELISAs. Detects human, mouse, and rat FABP2/I-FABP in Western blots.

Source

Monoclonal Mouse IgG_2A Clone # 989821

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human FABP2/I-FABP
Met1-Asp132
Accession # P12104

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Immunohistochemistry

5-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human, Mouse, and Rat FABP2/I-FABP by Western Blot. Western blot shows lysates of human small intestine tissue, mouse small intestine tissue, and rat small intestine tissue. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat FABP2/I-FABP Monoclonal Antibody (Catalog # MAB30782) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for FABP2/I-FABP at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

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FABP2/I‑FABP in Human Small Intestine. FABP2/I-FABP was detected in immersion fixed paraffin-embedded sections of human small intestine using Mouse Anti-Human/Mouse/Rat FABP2/I-FABP Monoclonal Antibody (Catalog # MAB30782) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FABP2/I-FABP

Intestinal Fatty Acid Binding Protein (FABP2), also known as I-FABP or gFABP is a member of the fatty acid binding protein family of intracellular proteins. FABP2 is only expressed in intestine, and it mediates the absorption and intracellular transport of dietary long-chain fatty acids. Genetic variation of FABP2 is implicated in obesity and Type II diabetes.

Long Name

Fatty Acid-Binding Protein 2/Intestinal FABP

Entrez Gene IDs

2169 (Human); 25598 (Rat)

Alternate Names

FABP2; FABPI; fatty acid binding protein 2, intestinal; Fatty acid-binding protein 2; fatty acid-binding protein, intestinal; IFABP; I-FABP; I-FABPMGC133132; Intestinal FABP; Intestinal-type fatty acid-binding protein