Human/Mouse/Rat TDP-43/TARDBP Antibody

Catalog # Availability Size / Price
MAB77781-SP Contact your distributor for availability.25 ug / Please Inquire
MAB77781-100 Contact your distributor for availability.100 ug / Please Inquire
Detection of Human, Mouse, and Rat TDP-43/TARDBP by Western Blot.
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Human/Mouse/Rat TDP-43/TARDBP Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human TDP-43/TARDBP in direct ELISAs. Detects human, mouse, and rat TDP-43/TARDBP in Western blots.
Source
Monoclonal Mouse IgG2A Clone # 982022
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E.coli-derived recombinant human TDP-43/TARDBP
Met1-Thr103
Accession # Q13148
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
3-25 µg/mL
See below
Immunocytochemistry
3-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human, Mouse, and Rat TDP-43/TARDBP antibody by Western Blot. View Larger

Detection of Human, Mouse, and Rat TDP-43/TARDBP by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, K562 human chronic myelogenous leukemia cell line, RAW 264.7 mouse monocyte/macrophage cell line, and NR8383 rat alveolar macrophage cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat TDP-43/TARDBP Monoclonal Antibody (Catalog # MAB77781) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for TDP-43/TARDBP at approximately 43 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry TDP-43/TARDBP antibody in Mouse Splenocytes by Immunocytochemistry (ICC). View Larger

TDP-43/TARDBP in Mouse Splenocytes. TDP-43/TARDBP was detected in immersion fixed mouse splenocytes using Mouse Anti-Human/Mouse/Rat TDP-43/TARDBP Monoclonal Antibody (Catalog # MAB77781) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Immunohistochemistry TDP-43/TARDBP antibody in Human Brain by Immunohistochemistry (IHC-P). View Larger

TDP-43/TARDBP in Human Brain. TDP-43/TARDBP was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Mouse Anti-Human/Mouse/Rat TDP-43/TARDBP Monoclonal Antibody (Catalog # MAB77781) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TDP-43/TARDBP

TDP-43 (TAR DNA binding protein of 43 kDa; gene name TARDBP) is a 414 amino acid (aa) nuclear RNA/DNA binding phosphoprotein that regulates transcription and splicing. TDP-43 is aberrantly expressed in most forms of amylotrophic lateral sclerosis and frontotemporal lobar degeneration. In these cases it is absent from the nucleus and accumulates, along with a truncated 20-28 kDa form, in ubiquitin-containing inclusion bodies. TDP-43 can also influence CFTR exon 9 skipping in cystic fibrosis. Within aa 1-103, human and mouse TDP-43 share 100% aa sequence identity, and 99% aa identity with rat TDP-43.

Long Name
TAR DNA-binding Protein 43
Entrez Gene IDs
23435 (Human)
Alternate Names
ALS10; ALS10TAR DNA-binding protein-43; TAR DNA binding protein; TARDBP; TDP43; TDP-43; TDP-43TAR DNA-binding protein 43

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Human/Mouse/Rat TDP-43/TARDBP Antibody
By Anonymous on 03/16/2022
Application: IHC Sample Tested: Brain tissue Species: Mouse