Human/Mouse Semaphorin 6B Antibody

Catalog #: MAB2094 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB2094
MAB2094-SP

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse Semaphorin 6B Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse Semaphorin 6B in direct ELISAs and Western blots. In direct ELISAs and Western blots, 100% cross-reactivity with recombinant mouse (rm) Semaphorin 6B is observed. In direct ELISAs, no cross‑reactivity with recombinant human Semaphorin 3A, 3B, 6A, 6C, 6D, 7A, rmSemaphorin 3C, 3E, or 3F is observed.

Source

Monoclonal Mouse IgG₁ Clone # 254402

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Semaphorin 6B
Leu26-Ser603
Accession # Q9H3T3

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human Semaphorin 6B Fc Chimera (Catalog # 2094-S6) under non-reducing conditions only

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Semaphorin 6B

Semaphorin 6B (Sema6B) is a 120 kDa member of the Semaphorin family of axon guidance molecules (1‑4). The four known Class 6 semaphorins are type I transmembrane glycoproteins with Sema domains but without other domains, making them most like Class 1 invertebrate semaphorins in structure (1‑4). Amino acid (aa) identity of Class 6 semaphorins is around 40% overall, but 53‑64% within the Sema domain. Sema6B is expressed developmentally in subregions of the nervous system and muscle, and at low levels in most adult tissues (3, 4). Human Sema6B cDNA encodes a 25 aa signal sequence, a 579 aa extracellular domain (ECD) including the Sema domain, a 20 aa transmembrane sequence and a 263 aa cytoplasmic portion. A cytoplasmic proline-rich sequence interacts with the SH3 domain of the c-src signaling protein (4). Full-length Sema6B is thought to form disulfide-linked homodimers (4). Alternate exon splicing creates a 492 aa (presumably) secreted form (Sema6B.1), and a 657 aa form with a shortened cytoplasmic tail (Sema6B.2) (3). Human Sema6B ECD shows 94%, 94%, 96% and 89% aa identity with corresponding mouse, rat, bovine and canine sequences, respectively. Crystal structures of semaphorins reveal that the 500 aa Sema domain forms an integrin‑like seven-blade beta -propeller structure stabilized by 14 conserved cysteine residues (5). Sema6B is highly expressed in some glioblastoma and breast cancer cell lines. All-trans retinoic acid slows cancer cell growth and down-regulates Sema6B expression, probably via dimerization with peroxisome proliferator-activated receptors (PPAR) that have a response element on the Sema6B gene (3, 6, 7). Semaphorins transduce signals through transmembrane plexins, either directly, or by binding associated neuropilin receptors. Plexin-A4 binds Sema6A (high affinity) and 6B (low affinity) and mediates sympathetic ganglion axon-repulsion, independent of neuropilin-1 (8).

References

Neufeld, G. et al. (2005) Front. Biosci. 10:751.
Chedotal, A. et al. (2005) Cell Death Differ. 12:1044.
Correa, R. G. et al. (2001) Genomics 73:343.
Eckhardt, F. et al. (1997) Mol. Cell. Neurosci. 9:409.
Gherardi, E. et al. (2004) Curr. Opin. Struct. Biol. 14:669.
Collet, P. et al. (2004) Genomics 83:1141.
Murad, H. et al. (2006) Int. J. Oncol. 28:977.
Suto, F. et al. (2005) J. Neurosci. 25:3628.

Entrez Gene IDs

10501 (Human); 20359 (Mouse)

Alternate Names

sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6B; Sema6B; SEMAN; Semaphorin 6B; semaphorin VIB; semaphorin Z; semaphorin-6B; semaphorin-6Ba; SEMA-VIB; SEMAZ; SEM-SEMA-Y; UNQ1907/PRO4353