Human/Mouse THAP11 Antibody Summary
Gly226-Met314
Accession # Q96EK4
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
View Larger
Detection of Human THAP11 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse THAP11 Monoclonal Antibody (Catalog # MAB5727) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for THAP11 at approximately 47 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
View Larger
THAP11 in BG01V Human Stem Cells. THAP11 was detected in immersion fixed undifferentiated BG01V human embryonic stem cells using Mouse Anti-Human/Mouse THAP11 Monoclonal Antibody (Catalog # MAB5727) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
View Larger
Detection of Human THAP11 by Simple WesternTM. Simple Western lane view shows lysates of BG01V human embryonic stem cells, loaded at 0.2 mg/mL. A specific band was detected for THAP11 at approximately 56 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human/Mouse THAP11 Monoclonal Antibody (Catalog # MAB5727). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: THAP11
THAP11, also known as Ronin, is a 36-44 kDa member of the thanatos-associated protein family. It is ubiquitously expressed and through its repression of c-Myc transcription, blocks cell proliferation. Human THAP11 is 314 amino acids (aa) in length. It contains a DNA-binding THAP zinc-finger domain (aa 5-81), an NLS (aa 98-104), a polyGln region (aa 104-146), an Ala-rich segment (aa 197-221) and a C-terminal coiled-coil region (aa 255-305). THAP11 is phosphorylated on Thr25. Over aa 226-314, human and mouse THAP11 are 100% identical in amino acid sequence.
Product Datasheets
Citations for Human/Mouse THAP11 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
3
Citations: Showing 1 - 3
Filter your results:
Filter by:
-
Insulin Receptor Associates with Promoters Genome-wide and Regulates Gene Expression
Authors: Melissa L. Hancock, Rebecca C. Meyer, Meeta Mistry, Radhika S. Khetani, Alexandre Wagschal, Taehwan Shin et al.
Cell
-
Genomic Determinants of THAP11/ZNF143/HCFC1 Complex Recruitment to Chromatin.
Authors: Vinckevicius A, Parker JB, Chakravarti D.
Mol Cell Biol.
-
Host Cell Factor-1 Recruitment to E2F-Bound and Cell-Cycle-Control Genes Is Mediated by THAP11 and ZNF143
Authors: J. Brandon Parker, Hanwei Yin, Aurimas Vinckevicius, Debabrata Chakravarti
Cell Reports
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsReviews for Human/Mouse THAP11 Antibody
There are currently no reviews for this product. Be the first to review Human/Mouse THAP11 Antibody and earn rewards!
Have you used Human/Mouse THAP11 Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
