Human PDGF-AA Antibody

Catalog #: MAB221
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB221-SP
MAB221-500
MAB221-100

Cell Proliferation Induced by PDGF‑AA and Neutralization by Human PDGF‑AA Antibody.

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All PDGF-AA Antibodies

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Citations (2)

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Human PDGF-AA Antibody Summary

Species Reactivity

Human

Specificity

Detects human PDGF-AA in direct ELISAs. In direct ELISAs, approximately 20% cross-reactivity with recombinant human (rh) PDGF-AB is observed and no cross-reactivity with rhPDGF-BB, rhPlGF, rhVEGF206, rhVEGF B₁₈₆, rhVEGF C, rhVEGF D, or rhCTGF is observed.

Source

Monoclonal Mouse IgG₁ Clone # 114506

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human PDGF-AA
Ser87-Thr211
Accession # P04085

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration

Sample

Neutralization

Measured by its ability to neutralize PDGF‑AA-induced proliferation in the NR6R‑3T3 mouse fibroblast cell line. Raines, E. W. et al. (1985) Methods Enzymol. 109:749. The Neutralization Dose (ND₅₀) is typically 0.2-1.0 µg/mL in the presence of 200 ng/mL Recombinant Human PDGF‑AA.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Cell Proliferation Induced by PDGF‑AA and Neutralization by Human PDGF‑AA Antibody. Recombinant Human PDGF-AA (Catalog # 221-AA) stimulates proliferation in the NR6R-3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human PDGF-AA (200 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human PDGF-AA Monoclonal Antibody (Catalog # MAB221). The ND₅₀ is typically 0.2-1.0 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PDGF-AA

Platelet-derived growth factor (PDGF) was discovered as a major mitogenic factor present in serum but absent from plasma. It was found to be secreted from the alpha ‑granules of platelets activated during the coagulation of blood to form serum. Subsequent studies have demonstrated that PDGF is not one molecule but three, each a dimeric combination of two distinct but structurally related peptide chains designated A and B. The dimeric isoforms PDGF-AA, AB and BB are differentially expressed in various cell types and their effects are mediated through two distinct receptors, termed alpha and beta. Differences exist in isoform binding to each receptor. In general, PDGF isoforms are potent mitogens for connective tissue cells, including dermal fibroblasts, glial cells, arterial smooth muscle cells and some epithelial and endothelial cells. In addition to its activity as a mitogen, PDGF is chemotactic for fibroblasts, smooth muscle cells, neutrophils and mononuclear cells. Other reported activities for PDGF include stimulation of granule release by neutrophils and monocytes, facilitation of steroid synthesis by Leydig cells, stimulation of neutrophil phagocytosis, inhibition of natural killer (NK) cell activity, stimulation of collagen synthesis, modulation of thrombospondin expression and secretion, stimulation of collagenase activity and secretion, induction of contraction of rat aorta strips in vitro, and transient induction of T cell IL-2 secretion accompanied by a down-regulation of IL-4 and IFN-gamma production, temporary effects that may allow clonal expansion of antigen-activated B and T helper lymphocytes prior to differentiation. PDGF also appears to be ubiquitous in neurons throughout the CNS, where it is suggested to play an important role in neuron survival and regeneration, and in mediation of glial cell proliferation and differentiation.

Long Name

Platelet-derived Growth Factor AA

Entrez Gene IDs

5154 (Human); 25266 (Rat)

Alternate Names

PDGF1; PDGF-A; PDGFAA; PDGF-AA

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human PDGF-AA Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Acidic fibroblast growth factor underlies microenvironmental regulation of MYC in pancreatic cancer
Authors: Sohinee Bhattacharyya, Chet Oon, Aayush Kothari, Wesley Horton, Jason Link, Rosalie C. Sears et al.
Journal of Experimental Medicine
Development and validation of sandwich ELISA microarrays with minimal assay interference.
Authors: Gonzalez RM, Seurynck-Servoss SL, Crowley SA
J. Proteome Res., 2008-04-19;7(6):2406-14.
Species: Human
Sample Types: Serum
Applications: ELISA Microarray Development