Human PKR Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human PKR by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, K562 human chronic myelogenous leukemia cell line, and HEK293 human embryonic kidney cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human PKR Monoclonal Antibody (Catalog # MAB1980) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for PKR at approximately 74 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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PKR in HeLa Human Cell Line. PKR was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line stimulated with rhIFN-alpha (Catalog # 11110-1) using Mouse Anti-Human PKR Monoclonal Antibody (Catalog # MAB1980) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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Detection of Human PKR by Simple WesternTM. Simple Western lane view shows lysates of K562 human chronic myelogenous leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for PKR at approximately 73 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human PKR Monoclonal Antibody (Catalog # MAB1980). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PKR
The interferon (IFN)-induced PKR mediates inhibition of protein synthesis through phosphorylation of the alpha subunit of eukaryotic inititation factor 2 (elF2alpha) and is also involved in the induction of the IFN gene through the activation of the transcription factor NF-kappa B.
Product Datasheets
Citations for Human PKR Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 9
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EIF2AK2 Missense Variants Associated with Early Onset Generalized Dystonia
Authors: Demy J. S. Kuipers, Wim Mandemakers, Chin‐Song Lu, Simone Olgiati, Guido J. Breedveld, Christina Fevga et al.
Annals of Neurology
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A frameshift mutation in the murine Prkra gene causes dystonia and exhibits abnormal cerebellar development and reduced eIF2? phosphorylation
Authors: Burnett, SB;Culver, AM;Simon, TA;Rowson, T;Frederick, K;Palmer, K;Murray, SA;Davis, SW;Patel, RC;
bioRxiv : the preprint server for biology
Species: Human
Sample Types: Cell Lysates
Applications: Immunoprecipitation -
Regulation of PKR activation and apoptosis during oxidative stress by TRBP phosphorylation
Authors: B Ukhueduan, E Chukwurah, RC Patel
The international journal of biochemistry & cell biology, 2021-06-24;137(0):106030.
Species: Human
Sample Types: Cell Lysates
Applications: Immunoprecipitation, Western Blot -
Kurarinone from Sophora Flavescens Roots Triggers ATF4 Activation and Cytostatic Effects Through PERK Phosphorylation
Authors: Sakiko Nishikawa, Yuka Itoh, Muneshige Tokugawa, Yasumichi Inoue, Ken-ichi Nakashima, Yuka Hori et al.
Molecules
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The Noncoding RNA nc886 Regulates PKR Signaling and Cytokine Production in Human Cells
Authors: E Golec, L Lind, M Qayyum, AM Blom, BC King
J. Immunol., 2018-12-05;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Stress-induced TRBP phosphorylation enhances its interaction with PKR to regulate cellular survival
Authors: E Chukwurah, RC Patel
Sci Rep, 2018-01-18;8(1):1020.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Altered activation of protein kinase PKR and enhanced apoptosis in dystonia cells carrying a mutation in PKR activator protein PACT.
Authors: Vaughn L, Bragg D, Sharma N, Camargos S, Cardoso F, Patel R
J Biol Chem, 2015-07-31;290(37):22543-57.
Species: Human
Sample Types: Cell Lysates
Applications: Immunoprecipitation -
Essential role of PACT-mediated PKR activation in tunicamycin-induced apoptosis.
Authors: Singh M, Fowlkes V, Handy I, Patel CV, Patel RC
J. Mol. Biol., 2008-11-05;385(2):457-68.
Species: Human
Sample Types: Whole Cells
Applications: Immunoprecipitation -
Protective paraspeckle hyper-assembly downstream of TDP-43 loss of function in amyotrophic lateral sclerosis
Authors: Tatyana A. Shelkovnikova, Michail S. Kukharsky, Haiyan An, Pasquale Dimasi, Svetlana Alexeeva, Osman Shabir et al.
Molecular Neurodegeneration
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